Wilson J F, Barnett K
Department of Pharmacology and Therapeutics, University of Wales College of Medicine, Cardiff, UK.
Ann Clin Biochem. 1995 Nov;32 ( Pt 6):540-4. doi: 10.1177/000456329503200604.
Ethanol was assayed by an average of 200 participants in the UK National External Quality Assessment Scheme, in 26 samples of human serum containing 0.1% fluoride/oxalate and added ethanol from 0.2 to 4.5 g/L. Outliers greater than three standard deviations from the consensus mean for any sample were excluded. Data remaining were grouped by technique and the technique mean and standard deviation calculated. Inter-laboratory variation of 13 technique groups was assessed by the coefficient of variation of measurements and bias from the per cent difference of the technique mean from the target value. Gas chromatography (GC) with packed columns and Sigma alcohol dehydrogenase assay protocols that include a sample deproteinization step, showed better between-laboratory agreement but greater bias. The least variable techniques were headspace analysis with GC-packed columns, Kodak Ektachem, bioMérieux and DuPont aca assays. A significant negative bias was produced by Kodak Ektachem and a positive bias by the Lion alcometer which was the most variable technique.
在英国国家外部质量评估计划中，平均有200名参与者对含有0.1%氟化物/草酸盐的26份人血清样本进行乙醇含量测定，这些样本中添加了0.2至4.5 g/L的乙醇。对于任何样本，偏离共识均值超过三个标准差的异常值被排除。剩余数据按技术分组，并计算技术均值和标准差。通过测量变异系数以及技术均值与目标值的百分比差异产生的偏差来评估13个技术组的实验室间差异。配备填充柱的气相色谱法（GC）以及包括样本脱蛋白步骤的Sigma乙醇脱氢酶测定方案，显示出更好的实验室间一致性，但偏差更大。变异性最小的技术是使用GC填充柱的顶空分析、柯达Ektachem、生物梅里埃和杜邦aca测定法。柯达Ektachem产生了显著的负偏差，而最具变异性的技术——狮王牌酒精测量仪则产生了正偏差。
