Wilson J F, Tsanaclis L M, Barnett K
Department of Pharmacology and Therapeutics, University of Wales College of Medicine, Cardiff, U.K.
Ther Drug Monit. 1996 Dec;18(6):721-3. doi: 10.1097/00007691-199612000-00016.
The Syva Emit and Abbott TDx II kits for determination of methotrexate in serum were compared using data from 14 samples distributed by the United Kingdom National External Quality Assessment Scheme to a mean of 38 European laboratories. For methotrexate concentrations above 0.2 mumol/L, there was no significant difference in the bias or coefficient of variation of measurements between the two techniques. A significantly greater number of measurements by Syva Emit (6.9%) were rejected as outliers > 3 SD from the sample mean compared with Abbott TDx (1.3%). The lower sensitivity of the Syva Emit assay was evident in data reported for samples containing methotrexate concentrations below 0.2 mumol/L.
使用来自英国国家外部质量评估计划分发给38个欧洲实验室的14个样本的数据，对用于测定血清中甲氨蝶呤的Syva Emit试剂盒和雅培TDx II试剂盒进行了比较。对于甲氨蝶呤浓度高于0.2μmol/L的情况，两种技术在测量偏差或变异系数方面没有显著差异。与雅培TDx（1.3%）相比，Syva Emit测定中被作为离群值（超过样本均值3个标准差）剔除的测量值数量显著更多（6.9%）。在报告的含有甲氨蝶呤浓度低于0.2μmol/L的样本数据中，Syva Emit测定的较低灵敏度很明显。
