Milne R W, Gutkowska J
Lipoprotein and Atherosclerosis Group, University of Ottawa Heart Institute, Ontario, Canada.
Biochem Biophys Res Commun. 1996 Jan 26;218(3):665-9. doi: 10.1006/bbrc.1996.0119.
We demonstrate immunoreactive mouse atrial natriuretic factor (ANF) in the IgG fraction prepared from ascites or serum of mice bearing an anti-rat ANFArg101-Tyr126 hybridoma. The HPLC profile of the ANF demonstrates that the IgG fraction contains both mature ANFSer99-Tyr126 and the prohormone ANFAsn1-Tyr126. The presence of circulating prohormone in the hybridoma-bearing mice is not secondary to changes in fluid volume due to ascites formation. The anti-ANF antibody appears to protect the ANFAsnl-Tyr126 from both maturation and rapid receptor-mediated clearance. Our results suggest, therefore, that the ANFAsn1-Tyr126 can be secreted into a compartment that is accessible to antibody and that maturation of the ANF prohormone may be an extracellular event.
我们在从小鼠腹水或血清中制备的IgG组分中证实了免疫反应性小鼠心房利钠因子（ANF），这些小鼠携带有抗大鼠ANFArg101-Tyr126杂交瘤。ANF的高效液相色谱图表明，IgG组分同时含有成熟的ANFSer99-Tyr126和前体激素ANFAsn1-Tyr126。携带杂交瘤的小鼠体内循环前体激素的存在并非腹水形成导致的液体量变化的继发结果。抗ANF抗体似乎可保护ANFAsnl-Tyr126不发生成熟以及不被快速的受体介导清除。因此，我们的结果提示，ANFAsn1-Tyr126可分泌至抗体可及的区室，并且ANF前体激素的成熟可能是一个细胞外事件。
