Afify A S, Yamazaki Y, Kageyama Y, Yusa S, Ogawa Y, Okada T, Okuno H
National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology, Ibaraki, Japan.
Z Naturforsch C J Biosci. 1995 Sep-Oct;50(9-10):664-8. doi: 10.1515/znc-1995-9-1011.
Esterases in nine rat hepatic and hepatoma-derived cell lines and normal rat liver homogenate were detected by polyacrylamide gel electrophoresis coupled with active staining with alpha-naphthyl acetate or butyrate as a substrate. The esterase band patterns of the non-cancerous and oncogene-transformed cell lines were alike, but different from those of hepatoma cell lines and normal rat liver homogenate. The former groups of cells might have completely lost the characteristics of rat liver parenchymal cells, or else they might have their origin at cells other than liver parenchyma. The esterase patterns of the hepatoma cell lines (e.g., McA-RH7777) and the normal rat liver highly resembled with each other, exemplifying the slight biochemical deviation of cancer from normal cells. However, two-dimensional electrophoretogram for the McA-RH7777 cell line showed a prominent esterase spot (pI 6.0-M(r) 110 kDa) that was lacking in the normal liver. This result indicates that there is invariably some change in esterase expression between the cancer cells and the normal liver cells.
通过聚丙烯酰胺凝胶电泳，以α-萘乙酸酯或丁酸酯作为底物进行活性染色，检测了9种大鼠肝脏及肝癌衍生细胞系和正常大鼠肝脏匀浆中的酯酶。非癌性细胞系和癌基因转化细胞系的酯酶条带模式相似，但与肝癌细胞系和正常大鼠肝脏匀浆的不同。前一组细胞可能已完全丧失大鼠肝脏实质细胞的特征，或者它们可能起源于肝脏实质以外的细胞。肝癌细胞系（如McA-RH7777）的酯酶模式与正常大鼠肝脏高度相似，例证了癌细胞与正常细胞之间轻微的生化偏差。然而，McA-RH7777细胞系的二维电泳图谱显示出一个正常肝脏中不存在的突出酯酶斑点（pI 6.0 - M(r) 110 kDa）。这一结果表明，癌细胞与正常肝细胞之间的酯酶表达总是存在一些变化。
