den Butter G, Saunder A, Marsh D C, Belzer F O, Southard J H
Department of Surgery, University of Wisconsin, Madison 53792, USA.
Transpl Int. 1995;8(6):466-71. doi: 10.1007/BF00335599.
The University of Wisconsin (UW) solution consists of a relatively complex mixture of agents. In this study we compared simpler preservation solutions, namely, histidine-tryptophan-ketoglutarate (HTK) and phosphate-buffered sucrose (PBS) with different compositions of UW solution in the isolated perfused rabbit liver model. Livers were stored cold for 24 and 48 h. After 24 h of preservation, the amount of bile produced in UW-preserved livers was significantly greater (P < 0.05) than that in HTK-preserved livers. Also, there was less LDH released into the perfusate in UW-preserved livers. There was more edema and lower K +/Na+rations in HTK-preserved livers than in UW-preserved livers (all data P < 0.05). After 48 h of preservation, the differences between livers preserved in UW or HTK solution were less noticeable than at 24 h and bile production was similar. LDH and AST release were greater in HTK-preserved livers than in UW livers, but these differences were not statistically significant. Preservation in PBS for 48 h was worse than in either UW or HTK solution. Substitution of polyethylene glycol (PEG) for hydroxyethyl starch (HES) in 48-h UW-preserved livers was not effective. We conclude that solutions simpler in composition than UW solution may be effective in kidney transplantation but do not appear suitable for successful liver preservation.
威斯康星大学（UW）溶液由相对复杂的多种成分混合而成。在本研究中，我们在离体灌注兔肝模型中，将成分更简单的保存溶液，即组氨酸 - 色氨酸 - 酮戊二酸（HTK）和磷酸盐缓冲蔗糖（PBS）与不同成分的UW溶液进行了比较。肝脏在低温下保存24小时和48小时。保存24小时后，UW保存的肝脏产生的胆汁量显著多于（P < 0.05）HTK保存的肝脏。此外，UW保存的肝脏中释放到灌注液中的乳酸脱氢酶（LDH）较少。HTK保存的肝脏比UW保存的肝脏有更多的水肿和更低的钾离子/钠离子比值（所有数据P < 0.05）。保存48小时后，UW溶液或HTK溶液保存的肝脏之间的差异不如24小时时明显，胆汁生成相似。HTK保存的肝脏中LDH和天冬氨酸转氨酶（AST）的释放量高于UW保存的肝脏，但这些差异无统计学意义。PBS保存48小时的效果比UW或HTK溶液都差。在保存48小时的UW溶液中用聚乙二醇（PEG）替代羟乙基淀粉（HES）无效。我们得出结论，成分比UW溶液更简单的溶液在肾移植中可能有效，但似乎不适用于成功的肝脏保存。
