Marinoni E, Picca A, Scucchi L, Cosmi E V, Di Iorio R
2nd Department of Obstetrics and Gynecology, University La Sapienza, Rome, Italy.
Am J Reprod Immunol. 1995 Oct;34(4):213-8. doi: 10.1111/j.1600-0897.1995.tb00943.x.
The aim of the study was to determine the ET-1 localization on human placenta and fetal membranes and to compare its distribution between term and preterm pregnancies in laboring and non-laboring tissues.
Tissues obtained from nine term elective cesarean section, eight spontaneous vaginal term delivery, and 13 preterm delivery from both cesarean section (N = 6) and vaginal delivery (N = 7) were studied by immunohistochemistry.
Immunoreactive ET-1 (IR-ET-1) was detected in villous and nonvillous trophoblast in all groups, although laboring tissues showed strong staining in the syncytiotrophoblast of the villi. ET-1 immunostaining of endothelial cells was observed in all placental villous vessels with a considerable variability within groups. In the fetal membranes, intensive immunopositive staining was observed in the chorionic trophoblast following vaginal deliveries in term and preterm tissues.
This is the first study to report the localization of IR-ET-1 in human fetal membranes and placenta, and suggests that amnion and trophoblast represents a source of ET-1 production or, alternatively, a site for ET-1 binding.
本研究的目的是确定内皮素-1（ET-1）在人胎盘和胎膜上的定位，并比较其在足月和早产分娩及未分娩组织中的分布情况。
通过免疫组织化学研究了从9例足月择期剖宫产、8例足月自然阴道分娩以及13例早产（剖宫产6例，阴道分娩7例）获取的组织。
所有组的绒毛和非绒毛滋养层细胞中均检测到免疫反应性ET-1（IR-ET-1），不过分娩组织的绒毛合体滋养层细胞显示出强染色。在所有胎盘绒毛血管中均观察到内皮细胞的ET-1免疫染色，组内存在相当大的变异性。在胎膜中，足月和早产组织经阴道分娩后，在绒毛膜滋养层细胞中观察到强烈的免疫阳性染色。
这是首次报道IR-ET-1在人胎膜和胎盘中定位的研究，并表明羊膜和滋养层细胞是ET-1的产生来源，或者是ET-1的结合部位。
