Henriques C U, Rice G E, Wong M H, Bendtzen K
Department of Obstetrics and Gynaecology, Rigshospitalet (National University Hospital), Copenhagen, Denmark.
Gynecol Obstet Invest. 1998;46(3):172-7. doi: 10.1159/000010027.
The aim of this study was to characterise the localisation and staining intensity of immunoreactive (ir) interleukin-4 (IL-4) and IL-4 receptor (IL-4R) in human placenta and fetal membranes in association with pre-term and term labour, and pre-eclampsia. The data obtained in this study establish the presence of irIL-4 and IL-4R in human placenta and fetal membranes obtained from women at 25-41 completed weeks of gestation. IL-4 and IL-4R immunohistochemical staining was principally localised in villous and chorionic trophoblast, and to amnion epithelial cells. The intensity of IL-4 and IL-4R immunohistochemical staining was not significantly affected by labour status at term or pre-term (p > 0. 05), with the exception of IL-4R in the amnion (p < 0.05). In term amnion, IL-4R was only detectable following labour onset. When data were stratified with respect to the presence or absence of pre-eclampsia, no statistical difference in immunohistochemical localisation or staining intensity for either IL-4 or IL-4R could be identified for any of the tissues studied. Co-localisation of IL-4 and IL-4R within gestational tissues may indicate auto- and/or paracrine mechanisms of action for this cytokine. Tissue-specific, labour-associated induction of IL-4R may contribute to the regulation of biological effects of IL-4 within the uteroplacental unit.
本研究的目的是确定免疫反应性（ir）白细胞介素-4（IL-4）和IL-4受体（IL-4R）在人胎盘和胎膜中的定位及染色强度，及其与早产、足月产和子痫前期的关系。本研究获得的数据证实了在妊娠25 - 41周的妇女所获得的人胎盘和胎膜中存在irIL-4和IL-4R。IL-4和IL-4R免疫组化染色主要定位于绒毛和绒毛膜滋养层细胞以及羊膜上皮细胞。足月或早产时，IL-4和IL-4R免疫组化染色强度不受产程影响（p>0.05），但羊膜中的IL-4R除外（p<0.05）。在足月羊膜中，仅在临产开始后才能检测到IL-4R。当根据子痫前期的有无对数据进行分层时，在所研究的任何组织中，IL-4或IL-4R的免疫组化定位或染色强度均未发现统计学差异。IL-4和IL-4R在妊娠组织中的共定位可能表明该细胞因子的自分泌和/或旁分泌作用机制。IL-4R的组织特异性、与产程相关的诱导可能有助于调节子宫胎盘单位内IL-4的生物学效应。
