一项关于单次及同时间歇性给予人甲状旁腺激素（1-34）和双膦酸盐西马多酸盐对去卵巢大鼠胫骨近端干骺端影响的组织形态计量学研究。

A histomorphometric study on effects of single and concurrent intermittent administration of human PTH (1-34) and bisphosphonate cimadronate on tibial metaphysis in ovariectomized rats.

作者信息

Mashiba T, Tanizawa T, Takano Y, Takahashi H E, Mori S, Norimatsu H

机构信息

Department of Orthopaedic Surgery, Niigata University, School of Medicine, Japan.

出版信息

Bone. 1995 Oct;17(4 Suppl):273S-278S. doi: 10.1016/8756-3282(95)00303-u.

DOI:10.1016/8756-3282(95)00303-u

PMID:8579928

Abstract

This study compared the single administration of hPTH(1-34), bisphosphonate cimadronate (YM-175), and concurrent therapy of these two for restoration of lost bone mass in ovariectomized (OVX) rats. Animals were untreated for 4 weeks after surgery, and then injected s.c. with vehicle (OVX+V), hPTH(1-34) (30 micrograms/kg) (OVX+P), YM-175 (5 micrograms/kg) (OVX+Y), or a combination of these two (OVX+P+Y), 3 days a week, for 8 weeks, and sacrificed. Their proximal tibia were processed undecalcified for quantitative bone histomorphometry. Although OVX+Y showed a reduction of bone turnover compared to OVX+V, it failed to restore lost bone mass in OVX rats. In contrast, OVX+P exhibited a stimulation of bone formation and restored cancellous osteopenia due to OVX. OVX+P+Y also resulted a recovery of osteopenia, however, stimulation of bone formation by PTH was suppressed by YM-175.

摘要

本研究比较了单次给予人甲状旁腺激素（1-34）[hPTH(1-34)]、双膦酸盐西马唑来（YM-175）以及二者联合用药对去卵巢（OVX）大鼠骨量丢失恢复情况的影响。术后4周内动物不接受任何处理，随后每周3次皮下注射赋形剂（OVX+V）、hPTH(1-34)（30微克/千克）（OVX+P）、YM-175（5微克/千克）（OVX+Y）或二者联合制剂（OVX+P+Y），持续8周，之后处死动物。对其胫骨近端进行不脱钙处理，用于定量骨组织形态计量学分析。虽然与OVX+V组相比，OVX+Y组骨转换降低，但未能恢复OVX大鼠丢失的骨量。相反，OVX+P组表现出骨形成增加，并恢复了因去卵巢导致的松质骨减少。OVX+P+Y组也使骨质减少得到恢复，然而，YM-175抑制了PTH对骨形成的刺激作用。