Petite H, Duval J L, Frei V, Abdul-Malak N, Sigot-Luizard M F, Herbage D
Institut de Biologie et Chimie des Protéines, UPR CNRS 412, Lyon, France.
Biomaterials. 1995 Sep;16(13):1003-8. doi: 10.1016/0142-9612(95)94908-4.
Glutaraldehyde (GTA) is used to cross-link collagen-based biomaterials, but these materials are often cytotoxic. In order to overcome this problem, we have proposed the use of the acyl azide methods with either hydrazine or diphenylphosphoryl azide (DPPA) as reagents. In this paper we determine the cytocompatibility of acyl azide- and GTA-treated pericardium in vitro, by an organotypic chick aorta culture technique developed for the evaluation of the propensity of vascular cells (both endothelial and smooth muscle cells) to migrate and grow on the surface of biomaterials. We first examined pericardium stabilization as a function of GTA concentration and time, so that we could minimize residual GTA molecules in the material. Treatment for 72 h with 0.05% GTA was optimal for thermal stabilization of the pericardium with a denaturation temperature (Td) of 86.8 degrees C, providing similar results to treatment with 0.6% GTA for 4 h (Td = 85.1 degrees C). Pericardium treated in this way was, however, poorly cytocompatible with little vascular cell migration and growth when compared with tissues treated by the acyl azide methods. The best results were obtained with 0.5% DPPA; treated tissues showed a high level of cross-linking (Td = 82.4 degrees C) and three-fold increases in cell growth and migration over those in a non-toxic control.
戊二醛（GTA）用于交联基于胶原蛋白的生物材料，但这些材料往往具有细胞毒性。为了克服这个问题，我们提出使用酰基叠氮化物方法，以肼或二苯基磷酰叠氮化物（DPPA）作为试剂。在本文中，我们通过一种为评估血管细胞（内皮细胞和平滑肌细胞）在生物材料表面迁移和生长倾向而开发的器官型鸡主动脉培养技术，在体外确定了酰基叠氮化物和GTA处理的心包的细胞相容性。我们首先研究了心包稳定化与GTA浓度和时间的关系，以便我们能够将材料中残留的GTA分子降至最低。用0.05% GTA处理72小时对于心包的热稳定是最佳的，变性温度（Td）为86.8摄氏度，与用0.6% GTA处理4小时（Td = 85.1摄氏度）的结果相似。然而，与用酰基叠氮化物方法处理的组织相比，以这种方式处理的心包细胞相容性较差，血管细胞迁移和生长很少。用0.5% DPPA获得了最佳结果；处理过的组织显示出高水平的交联（Td = 82.4摄氏度），并且细胞生长和迁移比无毒对照增加了三倍。
