Guerrieri A, Sabbatini L, Zambonin P G, Ricci F, Pocobelli A, Cerulli L
Dipartimento di Chimica, Università della Basilicata, Potenza, Italy.
Biomaterials. 1995 Sep;16(13):1025-30. doi: 10.1016/0142-9612(95)94911-4.
The effect of some antidenaturant drugs on the formation of lysozyme deposits on soft contact lenses was investigated by in vitro experiments. Results were obtained which encourage the use of this strategy to reduce lens spoilage. At the same time emphasis is given to the possibility that some functionalities (i.e. lysine groups) present in drug molecules could promote protein adsorption. In order to evaluate at a quantitative level the effectiveness of the drugs used to minimize the formation of deposits on soft contact lenses, a selective method for the determination of adsorbed lysozyme was developed. It is based on the hydrolysis of the 'lens/adsorbed lysozyme' system, followed by high performance liquid chromatography coupled to electrochemical detection of the released tryptophan residues. The lysozyme calibration curve was linear up to 5 mg ml-1 and the detection limit was 5 ng injected.
通过体外实验研究了某些抗变性剂药物对溶菌酶在软性隐形眼镜上沉积形成的影响。获得的结果鼓励采用这种策略来减少镜片损坏。同时强调了药物分子中存在的某些官能团（即赖氨酸基团）可能促进蛋白质吸附的可能性。为了定量评估用于最小化软性隐形眼镜上沉积物形成的药物的有效性，开发了一种测定吸附溶菌酶的选择性方法。它基于“镜片/吸附的溶菌酶”系统的水解，然后是高效液相色谱与释放的色氨酸残基的电化学检测相结合。溶菌酶校准曲线在高达5 mg/ml时呈线性，检测限为进样5 ng。
