Castillo E J, Koenig J L, Anderson J M, Lo J
Biomaterials. 1985 Sep;6(5):338-45. doi: 10.1016/0142-9612(85)90089-4.
Lysozyme was adsorbed on spin cast and lathe cut soft contact lenses of poly-2-hydroxyethylmethacrylate (PHEMA) and on poly-HEMA-methacrylic acid (PHEMA/MAA). The in vitro adsorption process was followed by ATR-FTIR. Lysozyme adsorbs both, reversibly and irreversibly, on the surfaces. While the reversible bound lysozyme experiences only minor changes in its secondary structure, conformational changes occur for the irreversibly adsorbed protein. The type and extent of structural changes depend on the degree of protein coverage on the lens surface, as well as the chemical structure and surface morphology of the lenses. PHEMA/MAA lenses adsorbed thirty times more lysozyme than either of the PHEMA lenses. Fabrication processes appear to induce different adsorption behaviour, PHEMA lathe cut lenses adsorb twice the amount of protein compared with PHEMA spin cast lenses.
溶菌酶吸附在通过旋铸法和车床切割法制备的聚甲基丙烯酸2-羟乙酯（PHEMA）软性隐形眼镜以及聚甲基丙烯酸2-羟乙酯-甲基丙烯酸（PHEMA/MAA）上。体外吸附过程通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）进行监测。溶菌酶在这些表面上存在可逆和不可逆两种吸附方式。虽然可逆结合的溶菌酶二级结构仅发生微小变化，但不可逆吸附的蛋白质会发生构象变化。结构变化的类型和程度取决于镜片表面蛋白质的覆盖程度，以及镜片的化学结构和表面形态。PHEMA/MAA镜片吸附的溶菌酶量是PHEMA镜片的30倍。制造工艺似乎会引发不同的吸附行为，PHEMA车床切割镜片吸附的蛋白质量是PHEMA旋铸镜片的两倍。
