平面支撑双分子层中电场诱导的浓度梯度

Groves J T, Boxer S G

Department of Chemistry, Stanford University, California 94305-5080, USA.

Biophys J. 1995 Nov;69(5):1972-5. doi: 10.1016/S0006-3495(95)80067-6.

A simple method of generating electric field-induced concentration gradients in planar supported bilayers has been developed. Gradients of charged, fluorescently labeled probes were visualized by epifluorescence microscopy and could be observed at field strengths as low as 1 V/cm. Steady-state concentration gradients can be described by a simple competition between random diffusion and electric field-induced drift. A model based on this principle has been used to determine the diffusion coefficient of the fluorescent probes. This technique achieves a degree of electrical manipulation of supported bilayers that offers a variety of possibilities for the development of new molecular architectures and the study of biological membranes.

一种在平面支撑双层膜中产生电场诱导浓度梯度的简单方法已被开发出来。通过落射荧光显微镜可以观察到带电的、荧光标记探针的梯度，并且在低至1 V/cm的场强下就能观察到。稳态浓度梯度可以通过随机扩散和电场诱导漂移之间的简单竞争来描述。基于这一原理的模型已被用于确定荧光探针的扩散系数。这项技术实现了对支撑双层膜的一定程度的电操控，为新型分子结构的开发和生物膜的研究提供了多种可能性。