Vorndran C, Minta A, Poenie M
Department of Zoology, University of Texas at Austin 78712-1064, USA.
Biophys J. 1995 Nov;69(5):2112-24. doi: 10.1016/S0006-3495(95)80082-2.
A new family of fluorescent calcium indicators has been developed based on a new analog of BAPTA called FF6. This new BAPTA analog serves as a versatile synthetic intermediate for developing Ca2+ indicators targeted to specific intracellular environments. Two of these new Ca2+ indicators, fura-PE3 and fura-FFP18, are described in this report. Fura-PE3 is a zwitterionic indicator that resists the rapid leakage and compartmentalization seen with fura-2 and other polycarboxylate calcium indicators. In contrast to results obtained with fura-2, cells loaded with PE3 remain brightly loaded and responsive to changes in concentration of cytosolic free calcium for hours. Fura-FFP18 is an amphipathic indicator that to binds to liposomes and to cell membranes. Studies to be detailed later indicate that FFP18 functions as a near-membrane Ca2+ indicator and that calcium levels near the plasma membrane rise faster and higher than in the cytosol.
基于一种名为FF6的新型BAPTA类似物，已开发出一个新的荧光钙指示剂家族。这种新型BAPTA类似物作为一种通用的合成中间体，用于开发针对特定细胞内环境的Ca2+指示剂。本报告描述了其中两种新型Ca2+指示剂，即fura-PE3和fura-FFP18。Fura-PE3是一种两性离子指示剂，可抵抗fura-2和其他多羧酸盐钙指示剂所出现的快速泄漏和区室化现象。与使用fura-2获得的结果相反，加载了PE3的细胞在数小时内仍保持明亮的加载状态，并对胞质游离钙浓度的变化做出反应。Fura-FFP18是一种两亲性指示剂，可与脂质体和细胞膜结合。稍后将详细介绍的研究表明，FFP18作为一种近膜Ca2+指示剂发挥作用，并且质膜附近的钙水平比细胞质中升高得更快、更高。
