Morimoto M, Numata K, Tanaka K
Third Department of Internal Medicine, Yokohama City University School of Medicine, Japan.
J Gastroenterol Hepatol. 1995 Nov-Dec;10(6):655-61. doi: 10.1111/j.1440-1746.1995.tb01366.x.
Deoxythymidine kinase (TK; EC 2.7.1.21) activity in the liver has been used as a marker of liver regeneration after partial hepatectomy. In this study we examined TK activity of various organs, plasma and peripheral blood mononuclear cells (PMNC) in 70% partially hepatectomized rats. TK activity of lymph nodes, small intestine, heart, lung, kidney and thymus did not increase significantly during the course of the study, except for spleen at 72 h. On the other hand, PMNC-TK and liver cystosolic TK activity increased in a parallel fashion at all times after partial hepatectomy; they began to increase 12 h after surgery and peaked 48 h post-surgery. Fractionation of PMNC into T cells and B cells revealed that both populations increased and peaked 48 h post-surgery. Plasma TK peaked 12-24 h after surgery, then declined at 36, 48 and 72 h after partial hepatectomy. This change paralleled plasma levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT). PMNC-TK activity correlated significantly with liver cystosolic TK activity 24 h (r = 0.743; P < 0.05) and 48 h (r = 0.708; P < 0.05) after partial hepatectomy. However, it did not correlate with plasma levels of TK, AST and ALT. The results indicate that in the early stage of liver regeneration PMNC-TK may provide a marker of liver regenerative processes.
脱氧胸苷激酶（TK；EC 2.7.1.21）在肝脏中的活性已被用作部分肝切除术后肝脏再生的标志物。在本研究中，我们检测了70%部分肝切除大鼠的各种器官、血浆和外周血单个核细胞（PMNC）中的TK活性。在研究过程中，淋巴结、小肠、心脏、肺、肾和胸腺的TK活性没有显著增加，除了72小时时的脾脏。另一方面，部分肝切除术后，PMNC-TK和肝脏胞质TK活性始终呈平行增加；它们在手术后12小时开始增加，并在手术后48小时达到峰值。将PMNC分为T细胞和B细胞显示，这两个群体均在手术后48小时增加并达到峰值。血浆TK在手术后12 - 24小时达到峰值，然后在部分肝切除术后36、48和72小时下降。这种变化与血浆天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平平行。部分肝切除术后24小时（r = 0.743；P < 0.05）和48小时（r = 0.708；P < 0.05），PMNC-TK活性与肝脏胞质TK活性显著相关。然而，它与血浆TK、AST和ALT水平无关。结果表明，在肝脏再生的早期阶段，PMNC-TK可能提供肝脏再生过程的一个标志物。
