Tsuchiya T, Ikarashi Y, Arai T, Ohhashi J, Nakamura A
National Institute of Health Sciences, Tokyo, Japan.
J Appl Biomater. 1994 Winter;5(4):361-7. doi: 10.1002/jab.770050412.
Modified Eagle's minimum essential medium supplemented with 5% fetal calf serum was highly sensitive to cytotoxicity and formed large control colonies in the V79 colony assay. A highly sensitive cytotoxicity test was developed using 96-well microtiter plates. Test chemicals or extracts of polyurethane materials containing the same chemicals were added 24 h after inoculation of cell suspensions. The cells were fixed and stained with crystal violet after additional culture for 6 days (V79 cells) or 10 days (Balb/3T3 cells). In terms of sensitivity and rapid quantitative measurement, this modified colony microassay, using a low cell density in 96-well microplates, was superior to various cytotoxicity tests such as colony, growth inhibition, cytolethality, and agar diffusion assays.
添加5%胎牛血清的改良伊格尔最低必需培养基对细胞毒性高度敏感，并且在V79集落试验中形成了大量对照集落。使用96孔微量滴定板开发了一种高度敏感的细胞毒性试验。在接种细胞悬液24小时后，加入受试化学品或含有相同化学品的聚氨酯材料提取物。在再培养6天（V79细胞）或10天（Balb/3T3细胞）后，将细胞固定并用结晶紫染色。就敏感性和快速定量测量而言，这种在96孔微量滴定板中使用低细胞密度的改良集落微量试验优于各种细胞毒性试验，如集落试验、生长抑制试验、细胞致死性试验和琼脂扩散试验。
