De Camilli P, Takei K, McPherson P S
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
Curr Opin Neurobiol. 1995 Oct;5(5):559-65. doi: 10.1016/0959-4388(95)80059-x.
Temperature-sensitive shibire mutants of Drosophila melanogaster become rapidly paralyzed upon a shift to the restrictive temperature, which is due to a block in synaptic vesicle endocytosis. The shibire gene encodes the GTPase dynamin. Recent studies have shown that dynamin forms rings at the neck of invaginated clathrin-coated pits, and have suggested that a conformational change in the ring, which correlates with GTP hydrolysis, plays an essential role in vesicle fission.
黑腹果蝇的温度敏感型发动蛋白突变体在转移到限制温度后会迅速麻痹，这是由于突触小泡内吞作用受阻所致。发动蛋白基因编码GTP酶发动蛋白。最近的研究表明，发动蛋白在深陷的网格蛋白包被小窝颈部形成环，并表明环中的构象变化与GTP水解相关，在小泡分裂中起重要作用。
