乙醇利用调节蛋白：序列比对未发现其起源于醛脱氢酶和醇脱氢酶基因融合的证据。
Ethanol utilization regulatory protein: profile alignments give no evidence of origin through aldehyde and alcohol dehydrogenase gene fusion.
作者信息
Nicholas H B, Persson B, Jörnvall H, Hempel J
机构信息
Pittsburgh Supercomputing Center, Pennsylvania 15213, USA.
出版信息
Protein Sci. 1995 Dec;4(12):2621-4. doi: 10.1002/pro.5560041221.
Abstract
The suggestion that the ethanol regulatory protein from Aspergillus has its evolutionary origin in a gene fusion between aldehyde and alcohol dehydrogenase genes (Hawkins AR, Lamb HK, Radford A, Moore JD, 1994, Gene 146:145-158) has been tested by profile analysis with aldehyde and alcohol dehydrogenase family profiles. We show that the degree and kind of similarity observed between these profiles and the ethanol regulatory protein sequence is that expected from random sequences of the same composition. This level of similarity fails to support the suggested gene fusion.
摘要
有人提出，来自曲霉的乙醇调节蛋白在进化上起源于醛脱氢酶基因和醇脱氢酶基因之间的基因融合（霍金斯·AR、兰姆·HK、拉德福德·A、摩尔·JD，1994年，《基因》146:145 - 158），我们通过醛脱氢酶和醇脱氢酶家族图谱分析对这一观点进行了验证。我们发现，这些图谱与乙醇调节蛋白序列之间观察到的相似程度和类型，与相同组成的随机序列所预期的情况相符。这种相似水平无法支持所提出的基因融合观点。
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本文引用的文献
1
Pattern recognition in genetic sequences.基因序列中的模式识别。
Proc Natl Acad Sci U S A. 1979 Jul;76(7):3041. doi: 10.1073/pnas.76.7.3041.
2
Dual relationships of xylitol and alcohol dehydrogenases in families of two protein types.两种蛋白质类型家族中木糖醇与醇脱氢酶的双重关系。
FEBS Lett. 1993 Jun 7;324(1):9-14. doi: 10.1016/0014-5793(93)81522-2.
3
Aldehyde dehydrogenases: widespread structural and functional diversity within a shared framework.醛脱氢酶：共享框架内广泛的结构和功能多样性
Protein Sci. 1993 Nov;2(11):1890-900. doi: 10.1002/pro.5560021111.
4
Issues in searching molecular sequence databases.搜索分子序列数据库中的问题。
Nat Genet. 1994 Feb;6(2):119-29. doi: 10.1038/ng0294-119.
5
Evolution of transcription-regulating proteins by enzyme recruitment: molecular models for nitrogen metabolite repression and ethanol utilisation in eukaryotes.通过酶招募实现转录调节蛋白的进化：真核生物中氮代谢物阻遏和乙醇利用的分子模型
Gene. 1994 Sep 2;146(2):145-58. doi: 10.1016/0378-1119(94)90287-9.
6
A super-family of medium-chain dehydrogenases/reductases (MDR). Sub-lines including zeta-crystallin, alcohol and polyol dehydrogenases, quinone oxidoreductase enoyl reductases, VAT-1 and other proteins.中链脱氢酶/还原酶（MDR）超家族。亚系包括ζ-晶状体蛋白、醇脱氢酶和多元醇脱氢酶、醌氧化还原酶烯酰还原酶、VAT-1及其他蛋白质。
Eur J Biochem. 1994 Nov 15;226(1):15-22. doi: 10.1111/j.1432-1033.1994.tb20021.x.
7
Short-chain dehydrogenases/reductases (SDR).短链脱氢酶/还原酶（SDR）
Biochemistry. 1995 May 9;34(18):6003-13. doi: 10.1021/bi00018a001.
8
Identification of common molecular subsequences.常见分子子序列的鉴定
J Mol Biol. 1981 Mar 25;147(1):195-7. doi: 10.1016/0022-2836(81)90087-5.
9
Characteristics of alcohol/polyol dehydrogenases. The zinc-containing long-chain alcohol dehydrogenases.醇/多元醇脱氢酶的特性。含锌的长链醇脱氢酶。
Eur J Biochem. 1987 Sep 1;167(2):195-201. doi: 10.1111/j.1432-1033.1987.tb13323.x.
10
Profile analysis.轮廓分析
Methods Enzymol. 1990;183:146-59. doi: 10.1016/0076-6879(90)83011-w.
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