关节炎支原体与宿主组织抗原发生交叉反应的抗原来源研究。

Investigation into the origin of the antigens of Mycoplasma arthritidis cross-reacting with host tissue antigens.

作者信息

Tangen G, Kirchhoff H

机构信息

Institut für Mikrobiologie und Tierseuchen, Tierärztliche Hochschule Hannover, Germany.

出版信息

FEMS Immunol Med Microbiol. 1995 Sep;12(1):9-15. doi: 10.1111/j.1574-695X.1995.tb00168.x.

DOI:10.1111/j.1574-695X.1995.tb00168.x

PMID:8580906

Abstract

The electrophoretical separations of Mycoplasma arthritidis and the serum used in the cultivation medium show a high number of protein bands with identical molecular weights. Proteins with molecular weights of 84, 72 and 52 kDa also appeared to be identical with proteins of Mycoplasma arthritidis in their antigenic properties as demonstrated by Western blotting with rat-anti-Mycoplasma arthritidis serum. The autoradiography of electrophoretically separated Mycoplasma arthritidis cells metabolically labeled with 35S-methionine and 35S-cysteine revealed that the proteins of Mycoplasma arthiritidis identical in molecular weight and antigenic structure with serum proteins are synthesized by Mycoplasma arthritidis, and represent true translation products.

摘要

关节炎支原体与培养基中所用血清的电泳分离显示出大量分子量相同的蛋白条带。通过用大鼠抗关节炎支原体血清进行蛋白质印迹分析表明，分子量为84、72和52 kDa的蛋白质在抗原特性上也与关节炎支原体的蛋白质相同。对用³⁵S-甲硫氨酸和³⁵S-半胱氨酸进行代谢标记的电泳分离的关节炎支原体细胞进行放射自显影，结果显示，关节炎支原体中合成的与血清蛋白分子量和抗原结构相同的蛋白质是真正的翻译产物。