Tanaka Y, Ohta A, Matsuo M, Sakamoto H
Graduate School of Science and Technology, Kobe University, Japan.
DNA Res. 1995 Jun 30;2(3):143-6. doi: 10.1093/dnares/2.3.143.
We cloned and sequenced the cDNA which encodes the Caenorhabditis elegans homologue (suf-1) of the Drosophila suppressor of forked (su(f)) gene product. The amino acid sequence deduced from the suf-1 cDNA shares extensive similarity with the su(f) and the human 77K subunit of the CstF, including the proline residues near the carboxy-terminus. Developmental Northern blot analysis showed that a 2.6-kb suf-1 transcript was expressed constitutively from L1 to young adult stages, suggesting it has a housekeeping function in vivo. Furthermore, this idea is consistent with the recent proposition that su(f) homologues play an essential role as a subunit of the cleavage stimulation factor (CstF) during polyadenylation of mRNA precursors.
我们克隆并测序了编码秀丽隐杆线虫叉状抑制因子（su(f)）基因产物同源物（suf-1）的cDNA。从suf-1 cDNA推导的氨基酸序列与su(f)及人CstF的77K亚基具有广泛的相似性，包括羧基末端附近的脯氨酸残基。发育Northern印迹分析表明，一个2.6 kb的suf-1转录本在从L1期到年轻成虫期持续表达，提示它在体内具有管家功能。此外，这一观点与最近提出的su(f)同源物在mRNA前体聚腺苷酸化过程中作为切割刺激因子（CstF）的一个亚基发挥重要作用的观点一致。
