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1
Chimeric human CstF-77/Drosophila Suppressor of forked proteins rescue suppressor of forked mutant lethality and mRNA 3' end processing in Drosophila.嵌合的人CstF-77/果蝇叉状蛋白抑制因子可挽救果蝇中叉状突变体致死性并参与mRNA 3'末端加工。
Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10593-8. doi: 10.1073/pnas.162191899. Epub 2002 Jul 29.
2
The suppressor of forked gene of Drosophila, which encodes a homologue of human CstF-77K involved in mRNA 3'-end processing, is required for progression through mitosis.果蝇的叉状基因抑制因子编码一种参与mRNA 3'末端加工的人类CstF - 77K同源物，它是有丝分裂进程所必需的。
Mech Dev. 1999 Apr;82(1-2):41-50. doi: 10.1016/s0925-4773(99)00011-8.
3
A polyadenylation factor subunit is the human homologue of the Drosophila suppressor of forked protein.一种聚腺苷酸化因子亚基是果蝇叉状蛋白抑制因子的人类同源物。
Nature. 1994 Dec 1;372(6505):471-4. doi: 10.1038/372471a0.
4
Autoregulation at the level of mRNA 3' end formation of the suppressor of forked gene of Drosophila melanogaster is conserved in Drosophila virilis.果蝇黑腹果蝇叉状基因抑制因子mRNA 3'末端形成水平的自调控在果蝇属中是保守的。
Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14302-7. doi: 10.1073/pnas.95.24.14302.
5
The suppressor of forked protein of Drosophila, a homologue of the human 77K protein required for mRNA 3'-end formation, accumulates in mitotically-active cells.果蝇叉状蛋白的抑制因子，是mRNA 3'末端形成所需的人类77K蛋白的同源物，在有丝分裂活跃的细胞中积累。
Mech Dev. 1998 Mar;72(1-2):53-63. doi: 10.1016/s0925-4773(98)00017-3.
6
The Drosophila homologue of the 64 kDa subunit of cleavage stimulation factor interacts with the 77 kDa subunit encoded by the suppressor of forked gene.切割刺激因子64 kDa亚基的果蝇同源物与叉状基因抑制子编码的77 kDa亚基相互作用。
Nucleic Acids Res. 2000 Jan 15;28(2):520-6. doi: 10.1093/nar/28.2.520.
7
Tissue-specific autoregulation of Drosophila suppressor of forked by alternative poly(A) site utilization leads to accumulation of the suppressor of forked protein in mitotically active cells.通过可变聚腺苷酸化位点利用对果蝇叉状抑制因子进行组织特异性自调控，导致叉状抑制因子蛋白在有丝分裂活跃细胞中积累。
RNA. 2000 Nov;6(11):1529-38. doi: 10.1017/s1355838200001266.
8
Characterization of a Drosophila homologue of the 160-kDa subunit of the cleavage and polyadenylation specificity factor CPSF.果蝇切割与聚腺苷酸化特异性因子CPSF 160-kDa亚基的同源物的特性分析
Mol Gen Genet. 1998 Apr;257(6):672-80. doi: 10.1007/s004380050696.
9
Developmental expression pattern of the Caenorhabditis elegans homologue of the Drosophila suppressor of forked gene.秀丽隐杆线虫中果蝇叉状基因抑制因子同源物的发育表达模式。
DNA Res. 1995 Jun 30;2(3):143-6. doi: 10.1093/dnares/2.3.143.
10
The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation.人切割-聚腺苷酸化特异性因子的160-kD亚基协调前体mRNA 3'末端的形成。
Genes Dev. 1995 Nov 1;9(21):2672-83. doi: 10.1101/gad.9.21.2672.

引用本文的文献

1
Cell Cycle Kinase Polo Is Controlled by a Widespread 3' Untranslated Region Regulatory Sequence in Drosophila melanogaster.果蝇细胞周期激酶 Polo 受广泛的 3'非翻译区调控序列控制。
Mol Cell Biol. 2019 Jul 16;39(15). doi: 10.1128/MCB.00581-18. Print 2019 Aug 1.
2
The structural basis of CstF-77 modulation of cleavage and polyadenylation through stimulation of CstF-64 activity.CstF-77 通过刺激 CstF-64 活性来调节切割和多聚腺苷酸化的结构基础。
Nucleic Acids Res. 2018 Dec 14;46(22):12022-12039. doi: 10.1093/nar/gky862.
3
Drosophila Symplekin localizes dynamically to the histone locus body and tricellular junctions.果蝇Symplekin动态定位于组蛋白基因座体和三细胞交界处。
Nucleus. 2014;5(6):613-25. doi: 10.4161/19491034.2014.990860.
4
Delineating the structural blueprint of the pre-mRNA 3'-end processing machinery.描绘 pre-mRNA 3'-端加工机制的结构蓝图。
Mol Cell Biol. 2014 Jun;34(11):1894-910. doi: 10.1128/MCB.00084-14. Epub 2014 Mar 3.
5
The conserved intronic cleavage and polyadenylation site of CstF-77 gene imparts control of 3' end processing activity through feedback autoregulation and by U1 snRNP.CstF-77 基因保守的内含子切割和多聚腺苷酸化位点通过反馈自动调节和 U1 snRNP 赋予 3' 端加工活性的控制。
PLoS Genet. 2013;9(7):e1003613. doi: 10.1371/journal.pgen.1003613. Epub 2013 Jul 11.
6
The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation.剪接刺激因子蛋白 CstF-64 的铰链域对于 CstF-77 的相互作用、核定位和多聚腺苷酸化是必不可少的。
J Biol Chem. 2010 Jan 1;285(1):695-704. doi: 10.1074/jbc.M109.061705. Epub 2009 Nov 3.
7
PAP- and GLD-2-type poly(A) polymerases are required sequentially in cytoplasmic polyadenylation and oogenesis in Drosophila.PAP型和GLD-2型聚腺苷酸聚合酶在果蝇的细胞质聚腺苷酸化和卵子发生过程中依次发挥作用。
Development. 2008 Jun;135(11):1969-79. doi: 10.1242/dev.021444. Epub 2008 Apr 23.
8
The structure of the CstF-77 homodimer provides insights into CstF assembly.CstF-77 同二聚体的结构为深入了解 CstF 组装提供了线索。
Nucleic Acids Res. 2007;35(13):4515-22. doi: 10.1093/nar/gkm458. Epub 2007 Jun 21.
9
Control of poly(A) polymerase level is essential to cytoplasmic polyadenylation and early development in Drosophila.对果蝇中聚腺苷酸聚合酶水平的调控对于细胞质聚腺苷酸化和早期发育至关重要。
EMBO J. 2002 Dec 2;21(23):6603-13. doi: 10.1093/emboj/cdf633.

本文引用的文献

1
The hiiragi gene encodes a poly(A) polymerase, which controls the formation of the wing margin in Drosophila melanogaster.日比野基因编码一种聚腺苷酸聚合酶，该酶控制黑腹果蝇翅缘的形成。
Dev Biol. 2001 May 1;233(1):137-47. doi: 10.1006/dbio.2001.0205.
2
hnRNP F influences binding of a 64-kilodalton subunit of cleavage stimulation factor to mRNA precursors in mouse B cells.异质性核糖核蛋白F影响切割刺激因子的一个64千道尔顿亚基与小鼠B细胞中mRNA前体的结合。
Mol Cell Biol. 2001 Feb;21(4):1228-38. doi: 10.1128/MCB.21.4.1228-1238.2001.
3
Tissue-specific autoregulation of Drosophila suppressor of forked by alternative poly(A) site utilization leads to accumulation of the suppressor of forked protein in mitotically active cells.通过可变聚腺苷酸化位点利用对果蝇叉状抑制因子进行组织特异性自调控，导致叉状抑制因子蛋白在有丝分裂活跃细胞中积累。
RNA. 2000 Nov;6(11):1529-38. doi: 10.1017/s1355838200001266.
4
Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors.人类前体信使核糖核酸切割因子II（m）含有酵母蛋白的同源物，并连接其他两种切割因子。
EMBO J. 2000 Nov 1;19(21):5895-904. doi: 10.1093/emboj/19.21.5895.
5
Pre-messenger RNA processing factors in the Drosophila genome.果蝇基因组中的信使前体RNA加工因子。
J Cell Biol. 2000 Jul 24;150(2):F37-44. doi: 10.1083/jcb.150.2.f37.
6
Complex protein interactions within the human polyadenylation machinery identify a novel component.人类聚腺苷酸化机制内复杂的蛋白质相互作用鉴定出一种新组分。
Mol Cell Biol. 2000 Mar;20(5):1515-25. doi: 10.1128/MCB.20.5.1515-1525.2000.
7
The Drosophila homologue of the 64 kDa subunit of cleavage stimulation factor interacts with the 77 kDa subunit encoded by the suppressor of forked gene.切割刺激因子64 kDa亚基的果蝇同源物与叉状基因抑制子编码的77 kDa亚基相互作用。
Nucleic Acids Res. 2000 Jan 15;28(2):520-6. doi: 10.1093/nar/28.2.520.
8
GFP-tagged balancer chromosomes for Drosophila melanogaster.用于黑腹果蝇的绿色荧光蛋白标记的平衡染色体。
Mech Dev. 1999 Nov;88(2):229-32. doi: 10.1016/s0925-4773(99)00174-4.
9
The Drosophila poly(A)-binding protein II is ubiquitous throughout Drosophila development and has the same function in mRNA polyadenylation as its bovine homolog in vitro.果蝇聚腺苷酸结合蛋白II在果蝇发育过程中普遍存在，并且在体外mRNA聚腺苷酸化过程中与它的牛同源物具有相同的功能。
Nucleic Acids Res. 1999 Oct 1;27(19):3771-8. doi: 10.1093/nar/27.19.3771.
10
3'-End processing of pre-mRNA in eukaryotes.真核生物中前体信使核糖核酸的3'末端加工
FEMS Microbiol Rev. 1999 Jun;23(3):277-95. doi: 10.1111/j.1574-6976.1999.tb00400.x.

嵌合的人CstF-77/果蝇叉状蛋白抑制因子可挽救果蝇中叉状突变体致死性并参与mRNA 3'末端加工。

Chimeric human CstF-77/Drosophila Suppressor of forked proteins rescue suppressor of forked mutant lethality and mRNA 3' end processing in Drosophila.

作者信息

Benoit Béatrice, Juge François, Iral Florence, Audibert Agnès, Simonelig Martine

机构信息

Génétique du Développement de la Drosophile, Institut de Génétique Humaine, 141 Rue de la Cardonille, 34396 Montpellier Cedex 5, France.

出版信息

Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10593-8. doi: 10.1073/pnas.162191899. Epub 2002 Jul 29.

DOI:10.1073/pnas.162191899
PMID:12149458
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC124984/
Abstract

The Suppressor of forked [Su(f)] protein is the Drosophila homologue of CstF-77, a subunit of human cleavage stimulation factor (CstF) that is required for the first step of the mRNA 3' end processing reaction in vitro. We have addressed directly the role of su(f) in the mRNA 3' end processing reaction in vivo. We show that su(f) is required for the cleavage of pre-mRNA during mRNA 3' end formation. Analysis of the functional complementation between Su(f) and CstF-77 shows that most of the Drosophila protein (85%) can be exchanged for the human protein to produce chimeric CstF-77/Su(f) proteins that rescue lethality and cleavage defect during mRNA 3' end formation in su(f) mutants. Interestingly, we show that a domain in human CstF-77 is limiting for the rescue and that this domain is not able to reproduce protein interactions with the CstF subunits of Drosophila. We also show that chimeric CstF-77/Su(f) proteins that rescue lethality of su(f) mutants cannot restore utilization of a regulated poly(A) site in Drosophila. Taken together, these results demonstrate that CstF-77 and Su(f) have the same function in mRNA 3' end formation in vivo, but that these two proteins are not interchangeable for regulation of poly(A) site utilization.

摘要

叉状抑制因子[Su(f)]蛋白是人类切割刺激因子（CstF）的一个亚基CstF-77在果蝇中的同源物，CstF-77是体外mRNA 3'末端加工反应第一步所必需的。我们直接研究了su(f)在体内mRNA 3'末端加工反应中的作用。我们发现，在mRNA 3'末端形成过程中，su(f)是前体mRNA切割所必需的。对Su(f)和CstF-77之间功能互补性的分析表明，大部分果蝇蛋白（85%）可以被人类蛋白替代，从而产生嵌合的CstF-77/Su(f)蛋白，这些蛋白能够挽救su(f)突变体在mRNA 3'末端形成过程中的致死性和切割缺陷。有趣的是，我们发现人类CstF-77中的一个结构域在挽救过程中起限制作用，并且该结构域无法与果蝇的CstF亚基产生蛋白质相互作用。我们还表明，能够挽救su(f)突变体致死性的嵌合CstF-77/Su(f)蛋白不能恢复果蝇中一个受调控的聚腺苷酸化位点的使用。综上所述，这些结果表明CstF-77和Su(f)在体内mRNA 3'末端形成中具有相同的功能，但这两种蛋白在聚腺苷酸化位点使用的调控方面不可互换。