Nichols R, Lim I A
Department of Biological Chemistry, University of Michigan, Ann Arbor, MI 48109-1048, USA.
Cell Tissue Res. 1996 Jan;283(1):107-16. doi: 10.1007/s004410050518.
The spatial and temporal distribution of three peptides, DSK I, DSK II, and DSK 0, encoded by the Drosophila melanogaster drosulfakinin (Dsk) gene, have been examined in the central nervous system. DSK I and DSK II have a -RFamide C-terminus and are structurally similar to sulfakinin peptides; in contrast, DSK 0 contains -SFamide and is not structurally similar to sulfakinins. Antisera specificities were determined by the design of the antigens and confirmed by dot blot analysis and preincubation with peptides prior to their use in immunocytochemistry. The distribution of immunoreactivity suggests that all three DSK peptides are processed from the polypeptide precursor and expressed in many of the same cells. Expression was observed at all developmental stages with an increase in the level of staining and the number of immunoreactive cells as development progresses. Cells in the brain lobe, optic lobe, subesophageal ganglion, thoracic ganglia, and the eighth abdominal neuromere contain DSK-immunoreactive materials. Immunoreactive fibers project from some cells and extend into the brain and ventral ganglion with regions of extensive arborization. DSK 0 immunoreactivity provides initial evidence for the presence of a -SFamide peptide in neural tissue. The observed expression of DSK-immunoreactive materials throughout development in numerous cells of the central nervous system suggests that DSK peptides may serve as hormones, modulators, or transmitters involved in several functions.
已对果蝇（Drosophila melanogaster）速激肽（Dsk）基因编码的三种肽DSK I、DSK II和DSK 0在中枢神经系统中的时空分布进行了研究。DSK I和DSK II的C末端为-RFamide，在结构上与速激肽类似；相比之下，DSK 0含有-SFamide，在结构上与速激肽不同。抗血清特异性通过抗原设计确定，并在用于免疫细胞化学之前通过斑点印迹分析和与肽预孵育进行了确认。免疫反应性分布表明，所有三种DSK肽均由多肽前体加工而来，并在许多相同细胞中表达。在所有发育阶段均观察到表达，随着发育进程，染色水平和免疫反应性细胞数量增加。脑叶、视叶、咽下神经节、胸神经节和第八腹神经节中的细胞含有DSK免疫反应性物质。免疫反应性纤维从一些细胞伸出并延伸至脑和腹神经节，具有广泛的分支区域。DSK 0免疫反应性为神经组织中存在-SFamide肽提供了初步证据。在中枢神经系统众多细胞的整个发育过程中观察到的DSK免疫反应性物质的表达表明，DSK肽可能作为参与多种功能的激素、调节剂或递质。
