Nichols R, McCormick J, Lim I
Depart of Biological Chemistry, University of Michigan, Ann Arbor 48109-1048, USA.
Peptides. 1997;18(1):41-5. doi: 10.1016/s0196-9781(96)00279-3.
We have isolated TDVDHVFLRFamide (DMS), FDDYGHMRFamide (DSK), and DPKQDFMRFamide from Drosophila melanogaster. These peptides, structurally related by a common C-terminus -XRFamide, where X = L or M, are encoded by three different genes. To determine cellular expression, we have generated antisera to multiple antigenic peptides and performed double-label immunofluorescence using antisera raised in the same species host animal. Our results indicate that DMS and DSK immunoreactive materials have unique, non-overlapping expression patterns, while DMS and DPKQDFMRFamide immunoreactive materials colocalize in two superior protocerebrum neurons, and DSK and DPKQDFMRFamide immunoreactive materials colocalize in one superior protocerebrum neuron, one subesophageal ganglion neuron, and three thoracic ganglia neurons.
我们已经从黑腹果蝇中分离出了TDVDHVFLRFamide（DMS）、FDDYGHMRFamide（DSK）和DPKQDFMRFamide。这些肽在结构上通过一个共同的C末端-XRFamide相关联，其中X = L或M，由三个不同的基因编码。为了确定细胞表达情况，我们制备了针对多种抗原肽的抗血清，并使用在同一物种宿主动物中产生的抗血清进行了双标记免疫荧光实验。我们的结果表明，DMS和DSK免疫反应性物质具有独特的、不重叠的表达模式，而DMS和DPKQDFMRFamide免疫反应性物质在两个上原脑神经元中共定位，DSK和DPKQDFMRFamide免疫反应性物质在一个上原脑神经元、一个咽下神经节神经元和三个胸神经节神经元中共定位。
