环孢素诱导的高血压大鼠主动脉细胞中内皮素受体的基因表达。

Gene expression of endothelin receptors in aortic cells from cyclosporine-induced hypertensive rats.

Iwai J, Kanayama Y, Negoro N, Okamura M, Takeda T

First Department of Internal Medicine, Osaka City University Medical School, Japan.

Clin Exp Pharmacol Physiol. 1995 Jun-Jul;22(6-7):404-9. doi: 10.1111/j.1440-1681.1995.tb02030.x.

Abstract

We examined preproendothelin-1, ETA and ETB receptor mRNA levels in aortic endothelial and smooth muscle cells from cyclosporine (CyA)-induced hypertensive rats using the reverse transcription polymerase chain reaction method. 2. Aortic endothelial preproendothelin-1 mRNA expression was about 1.5-fold higher, while that of ETB receptor mRNA was markedly decreased in CyA-treated rats compared with those in controls. 3. The expression of ETA receptor mRNA in smooth muscle cells from CyA-induced hypertensive rats was increased about two-fold over that in cells from control animals. 4. Thus, increased endothelial preproendothelin-1, and ETA receptor mRNA levels in smooth muscle cells, which are concomitant with the decrease in ETB receptor mRNA levels in endothelium, may contribute to CyA-induced hypertension in rats.

摘要

我们采用逆转录聚合酶链反应法，检测了环孢素（CyA）诱导的高血压大鼠主动脉内皮细胞和平滑肌细胞中前内皮素-1、ETA和ETB受体mRNA水平。2. 与对照组相比，经CyA处理的大鼠主动脉内皮前内皮素-1 mRNA表达约高1.5倍，而ETB受体mRNA表达明显降低。3. CyA诱导的高血压大鼠平滑肌细胞中ETA受体mRNA的表达比对照动物细胞中的表达增加了约两倍。4. 因此，内皮前内皮素-1增加以及平滑肌细胞中ETA受体mRNA水平升高，同时内皮中ETB受体mRNA水平降低，可能是导致大鼠CyA诱导性高血压的原因。