Al-Khalifah Nasser S, Shanavaskhan A E
National Center for Agricultural Technology, King Abdulaziz City for Science and Technology, P.O. Box 6086, Riyadh, 11442, Saudi Arabia.
Methods Mol Biol. 2017;1638:185-196. doi: 10.1007/978-1-4939-7159-6_16.
Ambiguity in the total number of date palm cultivars across the world is pointing toward the necessity for an enumerative study using standard morphological and molecular markers. Among molecular markers, DNA markers are more suitable and ubiquitous to most applications. They are highly polymorphic in nature, frequently occurring in genomes, easy to access, and highly reproducible. Various molecular markers such as restriction fragment length polymorphism (RFLP), amplified fragment length polymorphism (AFLP), simple sequence repeats (SSR), inter-simple sequence repeats (ISSR), and random amplified polymorphic DNA (RAPD) markers have been successfully used as efficient tools for analysis of genetic variation in date palm. This chapter explains a stepwise protocol for extracting total genomic DNA from date palm leaves. A user-friendly protocol for RAPD analysis and a table showing the primers used in different molecular techniques that produce polymorphisms in date palm are also provided.
全球枣椰树栽培品种总数的不明确性表明，有必要使用标准的形态学和分子标记进行一项枚举研究。在分子标记中，DNA标记对大多数应用来说更合适且普遍存在。它们本质上具有高度多态性，频繁出现在基因组中，易于获取且具有高度可重复性。各种分子标记，如限制性片段长度多态性（RFLP）、扩增片段长度多态性（AFLP）、简单序列重复（SSR）、简单序列重复区间（ISSR）和随机扩增多态性DNA（RAPD）标记，已成功用作分析枣椰树遗传变异的有效工具。本章解释了从枣椰树叶片中提取总基因组DNA的逐步方案。还提供了一个便于用户操作的RAPD分析方案以及一个表格，展示了在不同分子技术中用于产生枣椰树多态性的引物。
