Baumgartner-Parzer S M, Wagner L, Pettermann M, Gessl A, Waldhäusl W
Department of Internal Medicine III, University of Vienna, Austria.
Diabetologia. 1995 Nov;38(11):1367-70. doi: 10.1007/BF00401771.
We evaluated the influence of high ambient glucose on cellular expression of adhesion molecules, known to mediate endothelial interaction of leucocytes and monocytes. Paired cultures of individual isolates of human umbilical vein endothelial cells (HUVECs) were studied by fluorescence activated cell sorter analysis after exposure to 30 vs 5 mmol/l glucose. Incubation of HUVECs for 24h in 30 mmol/l glucose increased ICAM-1 (intercellular adhesion molecule-1; 116.4 +/- 16.9% of control, p < or = 0.05), but not PECAM (platelet endothelial cell adhesion molecule) expression, compared to cultures kept in 5 mmol/l glucose. Long-term exposure (13 +/- 1 days) of HUVECs to 30 mmol/l glucose increased expression of ICAM-1 to 122.5 +/- 32.2% (p < 0.002) and reduced that of PECAM to 86.9 +/- 21.3% vs the respective control culture in 5 mmol/l glucose (p < 0.02). Stimulation of confluent HUVECs, kept in 30 vs 5 mmol/l glucose for 13 +/- 1 days, with 20 U/ml interleukin-1 for 24 h (ICAM-1) and 4 h (endothelial leukocyte adhesion molecule 1) resulted in reduced ICAM-1 (84.8 +/- 27.0%, p < 0.05) and endothelial leukocyte adhesion molecule-1 (87.6 +/- 22.4%, p < 0.05) expression vs control cells, while that of PECAM (t:24 h) and vascular cell adhesion molecule-1 (t: 16 h) remained unchanged. In conclusion, it appears that differences in expression of adhesion molecules on HUVECs in response to high glucose reflects endothelial glucose toxicity, which may also induce endothelial dysfunction in diabetes.
我们评估了高环境葡萄糖对粘附分子细胞表达的影响，已知这些粘附分子介导白细胞和单核细胞与内皮细胞的相互作用。在暴露于30 mmol/L与5 mmol/L葡萄糖后，通过荧光激活细胞分选分析对人脐静脉内皮细胞（HUVECs）单个分离株的配对培养物进行了研究。与置于5 mmol/L葡萄糖中的培养物相比，将HUVECs在30 mmol/L葡萄糖中孵育24小时可增加细胞间粘附分子-1（ICAM-1）的表达（为对照的116.4±16.9%，p≤0.05），但血小板内皮细胞粘附分子（PECAM）的表达未增加。将HUVECs长期暴露（13±1天）于30 mmol/L葡萄糖中，与置于5 mmol/L葡萄糖中的相应对照培养物相比，ICAM-1的表达增加至122.5±32.2%（p<0.002），而PECAM的表达降低至86.9±21.3%（p<0.02）。将在30 mmol/L与5 mmol/L葡萄糖中培养13±1天的融合HUVECs分别用20 U/ml白细胞介素-1刺激24小时（针对ICAM-1）和4小时（针对内皮白细胞粘附分子1），结果显示与对照细胞相比，ICAM-1（84.8±27.0%，p<0.05）和内皮白细胞粘附分子-1（87.6±22.4%，p<0.05）的表达降低，而PECAM（24小时）和血管细胞粘附分子-1（16小时）的表达保持不变。总之，似乎HUVECs上粘附分子表达对高葡萄糖的差异反映了内皮细胞葡萄糖毒性，这也可能在糖尿病中诱导内皮功能障碍。
