Ruardy T G, Schakenraad J M, van der Mei H C, Busscher H J
Laboratory for Materia Technica, University of Groningen, The Netherlands.
J Biomed Mater Res. 1995 Nov;29(11):1415-23. doi: 10.1002/jbm.820291113.
In this study, adhesion and spreading of human skin fibroblasts on gradient surfaces of dichlorodimethylsilane (DDS) coupled to glass was investigated. Gradient surfaces were prepared by the diffusion technique and characterized by the Wilhelmy plate technique and characterized by the Wilhelmy plate technique for their wettability and by scanning x-ray photoelectron spectroscopy for their chemical surface composition. A linear relation between the gradient length, based on advancing water contact angles, and the square root of the diffusion time of DDS was observed. XPS analysis and the cellular experiments were carried out on gradient surfaces prepared using a diffusion time of 3 h. A continuously varying chemical composition with Si/O elemental surface concentration ratio being highest on the hydrophobic end of the gradient surfaces. In the presence of serum proteins, human skin fibroblasts seeded on these gradient surfaces showed a preferential adhesion onto the steepest part of the gradient, probably due to an optimal local wettability and/or local chemistry. Furthermore, it was shown that the spread area of human fibroblasts increased over the length of the gradient surface when going from the hydrophobic to the hydrophilic end. Summarizing, this study shows that the use of gradient surfaces to study cellular responses to materials surface properties, like wettability, yields more-convincing conclusions than the use of a variety of materials with different wettabilities due to the control of the specific surface chemistry of gradient surfaces.
在本研究中，对人皮肤成纤维细胞在与玻璃偶联的二氯二甲基硅烷（DDS）梯度表面上的黏附与铺展进行了研究。通过扩散技术制备梯度表面，采用威尔海姆板技术对其润湿性进行表征，并利用扫描X射线光电子能谱对其化学表面组成进行表征。观察到基于前进水接触角的梯度长度与DDS扩散时间的平方根之间存在线性关系。XPS分析和细胞实验在使用3小时扩散时间制备的梯度表面上进行。梯度表面的化学成分呈连续变化，硅/氧元素表面浓度比在梯度表面的疏水端最高。在血清蛋白存在的情况下，接种在这些梯度表面上的人皮肤成纤维细胞表现出优先黏附在梯度最陡的部分，这可能是由于最佳的局部润湿性和/或局部化学性质。此外，研究表明，当从疏水端向亲水端移动时，人成纤维细胞的铺展面积在梯度表面的长度上增加。总之，本研究表明，与使用具有不同润湿性的多种材料相比，使用梯度表面来研究细胞对材料表面性质（如润湿性）的反应，由于能够控制梯度表面的特定表面化学性质，能得出更有说服力的结论。
