Bigelow M W, Wiessner J H, Kleinman J G, Mandel N S
Department of Medicine, Medical College of Wisconsin, Milwaukee, USA.
J Urol. 1996 Mar;155(3):1094-8.
Urolithiasis is clearly a multifaceted process, progressing from urine supersaturation to the formation of mature renal calculi. Retention of microcrystals by the urothelium is a critical event in stone maturation. Membrane phospholipids appear to be involved in the attachment of stone crystals to kidney epithelium.
The current study quantitates crystal-membrane interactions following selective changes in the red blood cell (RBC) membrane phospholipid composition by using a crystal-induced membranolytic assay.
Membrane enrichment with anionic phospholipids was found to greatly increase crystal-membrane interactions. Crystal-membrane interaction was associated with an increase in the negative charge on the RBC membrane surface.
Specific membrane compositions seem to facilitate the formation of crystal attachment region on the RBC surface that is necessary for effective crystal attachment to the cell membrane.
尿路结石显然是一个多方面的过程，从尿液过饱和发展到成熟肾结石的形成。尿路上皮对微晶的滞留是结石成熟过程中的关键事件。膜磷脂似乎参与了结石晶体与肾上皮的附着。
本研究通过晶体诱导的膜溶解试验，对红细胞（RBC）膜磷脂组成发生选择性变化后的晶体-膜相互作用进行定量分析。
发现富含阴离子磷脂的膜能显著增加晶体-膜相互作用。晶体-膜相互作用与红细胞膜表面负电荷增加有关。
特定的膜组成似乎有助于在红细胞表面形成晶体附着区域，这是晶体有效附着于细胞膜所必需的。
