白介素-2受体表达检测方法的建立及其在宽吻海豚（瓶鼻海豚）细胞免疫反应评估中的应用。

Development of an interleukin-2 receptor expression assay and its use in evaluation of cellular immune responses in bottlenose dolphin (Tursiops truncatus).

作者信息

Erickson K L, DiMolfetto-Landon L, Wells R S, Reidarson T, Stott J L, Ferrick D A

机构信息

Department of Veterinary Pathology, Microbiology, and Immunology, University of California, Davis 95616, USA.

出版信息

J Wildl Dis. 1995 Apr;31(2):142-9. doi: 10.7589/0090-3558-31.2.142.

DOI:10.7589/0090-3558-31.2.142

PMID:8583630

Abstract

We describe optimization of a peripheral blood mononuclear leukocyte proliferation assay and development of an interleukin-2 receptor (IL-2R) expression assay for bottlenose dolphins (Tursiops truncatus). Peripheral blood mononuclear leukocytes obtained from both Sea World (February 1993) and the Naval Command Control and Ocean Surveillance Center (March 1993) (San Diego, California, USA) were stimulated with the mitogens concanavalin A (ConA) and phytohemagglutinin (PHA) and evaluated for optimum proliferation and IL-2R expression. Based on these optimization assays, standard conditions were established and used to assess immune function in a population of apparently healthy, free-ranging bottlenose dolphins from Sarasota Bay, Florida (USA) in June 1993. A positive correlation was observed between proliferation assays using ConA and PHA as the stimulants. However, IL-2R expression induced by both mitogens differed significantly.

摘要

我们描述了宽吻海豚（Tursiops truncatus）外周血单个核白细胞增殖试验的优化以及白细胞介素-2受体（IL-2R）表达试验的开发。从海洋世界（1993年2月）和海军指挥控制与海洋监视中心（1993年3月）（美国加利福尼亚州圣地亚哥）获取的外周血单个核白细胞，用促细胞分裂剂刀豆球蛋白A（ConA）和植物血凝素（PHA）进行刺激，并评估其最佳增殖和IL-2R表达情况。基于这些优化试验，建立了标准条件，并于1993年6月用于评估来自美国佛罗里达州萨拉索塔湾一群看似健康、自由放养的宽吻海豚的免疫功能。观察到以ConA和PHA作为刺激物的增殖试验之间存在正相关。然而，两种促细胞分裂剂诱导的IL-2R表达存在显著差异。