Cao M Y, Deggouj N, Gersdorff M, Tomasi J P
Department of Otorhinolaryngology, Catholic University of Louvain, University Hospital of St-Luc, Brussels, Belgium.
Laryngoscope. 1996 Feb;106(2 Pt 1):207-12. doi: 10.1097/00005537-199602000-00019.
In this study, the authors attempted to develop a method of extracting guinea pig inner ear antigens for otoimmunological research, and to investigate the distribution of the antigens in the various structures of the inner ear. The antigens were extracted either from the entire or from various parts of the guinea pig inner ear. These antigens were separated on sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels. Western blot techniques were used to test sera from patients with inner ear disease against guinea pig inner ear protein extracts. It found that the various molecular weight antigens in the inner ear were associated with the different structures of the inner ear. The sera of 37.5% (N = 80) of patients reacted with two bands (30 and 58 kd) of the guinea pig inner ear immunoblots. The 58 kd band was not specific to the inner ear, but instead was also found in the immunoblots of other guinea pig tissues (brain, lung, and liver). This study suggests that the various antigens of interest could be further extracted and purified from the corresponding locations of the inner ear.
在本研究中，作者试图开发一种用于耳免疫学研究的豚鼠内耳抗原提取方法，并研究这些抗原在内耳各结构中的分布。抗原从豚鼠内耳的整体或各个部分提取。这些抗原在十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）凝胶上进行分离。采用蛋白质印迹技术，用内耳疾病患者的血清检测豚鼠内耳蛋白提取物。结果发现，内耳中不同分子量的抗原与内耳的不同结构相关。37.5%（N = 80）的患者血清与豚鼠内耳免疫印迹的两条带（30和58 kd）发生反应。58 kd的条带并非内耳所特有，在其他豚鼠组织（脑、肺和肝）的免疫印迹中也能发现。本研究表明，可从内耳相应部位进一步提取和纯化各种感兴趣的抗原。
