Cao M Y, Gersdorff M, Deggouj N, Tomasi J P
Department of Otorhinolaryngology, Université Catholique de Louvain, Cliniques Universitaires St-Luc, Brussels, Belgium.
J Laryngol Otol. 1995 Jan;109(1):19-23. doi: 10.1017/s0022215100129147.
In this study, we investigated the relative localization of some antigenic epitopes in the inner ear. The inner ear protein antigens were extracted from various parts of the guinea pig inner ear. Brain, kidney, lung, heart and liver extracts were also obtained. We found by SDS-polyacrylamide gel electrophoresis that total inner ear extracts separated into three high concentration polypeptide bands with molecular weights of approximately 30, 42, 58 kd and three low density bands of 20, 25 and 35 kd. The 30 kd band was found mainly in the extract of the spiral ganglion and the acoustic nerve in the modiolus. The 42 and 58 kd bands were detected in the extract of the spiral ligament and the stria vascularis. The Organ of Corti and the basilar membrane extract gave rise to three bands of 30, 42 and 58 kd. Twenty-eight of the 75 sera from patients with inner ear disease reacted with the 30 and 58 kd bands of the inner ear protein extracts by immunoblotting. Sixteen of these 28 positive sera were then used to probe immunoblots of the brain, kidney, lung, heart and liver extracts. The 58 kd band was also found in protein extracts of the brain, the lung and the liver. This study suggests that the 30 kd antigenic epitope may be mainly related to the acoustic nerve and that the 58 kd antigenic epitope is not cochlear specific.
在本研究中，我们调查了内耳中一些抗原表位的相对定位。内耳蛋白抗原从豚鼠内耳的各个部位提取。还获得了脑、肾、肺、心和肝的提取物。我们通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳发现，内耳总提取物分离为三条高浓度多肽带，分子量约为30、42、58kd，以及三条低密度带，分子量为20、25和35kd。30kd带主要存在于蜗轴螺旋神经节和听神经的提取物中。42和58kd带在螺旋韧带和血管纹的提取物中检测到。柯蒂氏器和基底膜提取物产生了30、42和58kd的三条带。75例内耳疾病患者的血清中有28例通过免疫印迹与内耳蛋白提取物的30和58kd带发生反应。然后，这28份阳性血清中的16份用于检测脑、肾、肺、心和肝提取物的免疫印迹。在脑、肺和肝的蛋白提取物中也发现了58kd带。本研究表明，30kd抗原表位可能主要与听神经有关，而58kd抗原表位并非耳蜗特异性的。
