Ghosh B, Barbosa E, Singh I
Department of Pediatrics, Medical University of South Carolina, Charleston 29425, USA.
Mol Cell Biochem. 1995 Oct 4;151(1):77-81. doi: 10.1007/BF01076899.
A complimentary DNA clone encoding the entire human palmitoyl-CoA ligase has been isolated from a liver cDNA library and sequenced in it's entirety. The predicted product is a 699 amino acid protein. Southern analysis utilizing the human palmitoyl-CoA ligase gene as a probe revealed varying degrees of similarity amongst various mammalian species. The palmitoyl-CoA ligase gene is highly expressed in liver, heart, skeletal muscle and kidney, and to a lesser extent in brain, lung, placenta and pancreas. The expression of palmitoyl-CoA ligase in various tissue parallels the function of this enzyme in the metabolism of fatty acids in these tissues.
一个编码整个人类棕榈酰辅酶A连接酶的互补DNA克隆已从肝脏cDNA文库中分离出来，并进行了全序列测定。预测的产物是一种含有699个氨基酸的蛋白质。利用人类棕榈酰辅酶A连接酶基因作为探针进行的Southern分析表明，各种哺乳动物物种之间存在不同程度的相似性。棕榈酰辅酶A连接酶基因在肝脏、心脏、骨骼肌和肾脏中高度表达，在大脑、肺、胎盘和胰腺中的表达程度较低。棕榈酰辅酶A连接酶在各种组织中的表达与该酶在这些组织中脂肪酸代谢的功能平行。
