Berlanga J J, Fresno Vara J A, Martín-Pérez J, García-Ruiz J P
Departamento de Biologia Molecular, Centro de Biologia Molecular Severo Ochoa, Madrid, Spain.
Mol Endocrinol. 1995 Nov;9(11):1461-7. doi: 10.1210/mend.9.11.8584023.
The mechanism of action of the pituitary hormone PRL was studied in hepatocytes of lactating rats. PRL receptor immune complexes obtained from liver lysates have an associated tyrosine kinase activity. The tyrosine kinase has been identified in isolated hepatocytes as pp60c-src. Incubation of hepatocytes with PRL induces the association of PRL receptor with pp60c-src and the resultant stimulation of its tyrosine kinase activity. Furthermore, PRL stimulates the gene expression of c-fos, c-jun, and c-src. All of these findings support the idea that the pp60c-src tyrosine kinase participates in the early steps of the PRL intracellular signaling that promotes cell growth in liver cells.
在泌乳大鼠的肝细胞中研究了垂体激素催乳素（PRL）的作用机制。从肝脏裂解物中获得的PRL受体免疫复合物具有相关的酪氨酸激酶活性。在分离的肝细胞中已鉴定出该酪氨酸激酶为pp60c-src。用PRL孵育肝细胞可诱导PRL受体与pp60c-src结合，并刺激其酪氨酸激酶活性。此外，PRL刺激c-fos、c-jun和c-src的基因表达。所有这些发现都支持这样一种观点，即pp60c-src酪氨酸激酶参与了PRL细胞内信号传导促进肝细胞生长的早期步骤。
