Ito M, Yamada K, Masuda J, Kinoshita A, Otsuki H, Hayakawa T
Department of Neurosurgery, Osaka University Medical School, Japan.
Neurol Res. 1995 Oct;17(5):345-8.
The platelet derived growth factor (PDGF) plays an important role for development of atherosclerosis. We therefore immunostained carotid atheroma specimens for PDGF. We also detected dividing cell species of the atheroma with in vitro labeling of bromodeoxyuridine (BUdR). Thirty specimens of carotid atheroma were obtained by endarterectomy and they were incubated for 3 hours with Dulbecco's modified Eagle medium/20% fetal calf serum culture medium containing BUdR/fluorodeoxyuridine (FUdR). They were ethanol-fixed, thin-sliced, and immunostained for BUdR, PDGF, smooth muscle actin and macrophage. The PDGF immunoreactivity was mainly detected in the macrophages of the subendothelial area, where BUdR-positive cells were present. Percentage of BUdR-positive cells in the atheroma specimens ranged from 3% to 15%. The BUdR-labeled small cells were mainly located in the subendothelial area, and they were identified as non-foamy macrophages by double immunostaining with anti-macrophage antibody. The results indicate that nonfoamy macrophages have potentials for cell division and they might play an important role for the development and growth of atheroma by secreting PDGF.
血小板衍生生长因子（PDGF）在动脉粥样硬化的发展过程中起着重要作用。因此，我们对颈动脉粥样瘤标本进行了PDGF免疫染色。我们还通过溴脱氧尿苷（BUdR）的体外标记检测了粥样瘤中的分裂细胞种类。通过动脉内膜切除术获取了30个颈动脉粥样瘤标本，并将它们与含有BUdR/氟脱氧尿苷（FUdR）的杜尔贝科改良伊格尔培养基/20%胎牛血清培养基一起孵育3小时。将它们用乙醇固定、切片，并对BUdR、PDGF、平滑肌肌动蛋白和巨噬细胞进行免疫染色。PDGF免疫反应性主要在存在BUdR阳性细胞的内皮下区域的巨噬细胞中检测到。粥样瘤标本中BUdR阳性细胞的百分比在3%至15%之间。BUdR标记的小细胞主要位于内皮下区域，通过用抗巨噬细胞抗体进行双重免疫染色，它们被鉴定为非泡沫巨噬细胞。结果表明，非泡沫巨噬细胞具有细胞分裂的潜能，并且它们可能通过分泌PDGF在粥样瘤的发展和生长中发挥重要作用。
