Agrawal R K, Penczek P, Grassucci R A, Li Y, Leith A, Nierhaus K H, Frank J
Wadsworth Center, New York State Department of Health, Albany 12201-0509, USA.
Science. 1996 Feb 16;271(5251):1000-2. doi: 10.1126/science.271.5251.1000.
Transfer RNA (tRNA) molecules play a crucial role in protein biosynthesis in all organisms. Their interactions with ribosomes mediate the translation of genetic messages into polypeptides. Three tRNAs bound to the Escherichia coli 70S ribosome were visualized directly with cryoelectron microscopy and three-dimensional reconstruction. The detailed arrangement of A- and P-site tRNAs inferred from this study allows localization of the sites for anticodon interaction and peptide bond formation on the ribosome.
转运核糖核酸（tRNA）分子在所有生物体的蛋白质生物合成中都起着至关重要的作用。它们与核糖体的相互作用介导了遗传信息向多肽的翻译。通过冷冻电子显微镜和三维重建直接观察到了与大肠杆菌70S核糖体结合的三种tRNA。从这项研究中推断出的A位点和P位点tRNA的详细排列，使得核糖体上反密码子相互作用位点和肽键形成位点得以定位。
