用于多头绒泡菌变形虫生长的限定培养基和半限定培养基。

Defined and semi-defined media for the growth of amoebae of Physarum polycephalum.

作者信息

McCullough C H, Dee J

出版信息

J Gen Microbiol. 1976 Jul;95(1):151-8. doi: 10.1099/00221287-95-1-151.

Abstract

Amoebae of the true slime mould Physarum polycephalum were cultured in two fully-defined liquid media containing amino acids, glucose, three vitamins and a buffered salts solution. Absolute requirements were demonstrated for methionine, haematin, thiamine and biotin, all of which were known to be specific requirements of the plasmodial stage. Methods are described for large-scale culture in three semi-defined media.

摘要

多头绒泡菌这种真正的黏菌的变形虫在两种完全确定的液体培养基中培养，这些培养基含有氨基酸、葡萄糖、三种维生素和一种缓冲盐溶液。已证明对蛋氨酸、血红素、硫胺素和生物素存在绝对需求，所有这些都是已知的原质团阶段的特定需求。文中描述了在三种半确定培养基中进行大规模培养的方法。