Gingold E C, Grant W D, Wheals A E, Wren M
Mol Gen Genet. 1976 Nov 24;149(1):115-9. doi: 10.1007/BF00275966.
Methods are described for the isolation and testing of temperature-sensitive plasmodial strains of Physarum polycephalum. Nineteen temperature-sensitive strains were found by screening plasmodia derived from mutagenised amoebae and the properties of these are described. A scheme is outlined for the detection of specific mitotic cycle lesions amongst temperature-sensitive strains, and the properties of a presumptive mitotic cycle mutant are described.
本文描述了多头绒泡菌温度敏感型原质团菌株的分离和测试方法。通过筛选诱变变形虫产生的原质团，发现了19个温度敏感型菌株，并描述了它们的特性。概述了一种检测温度敏感型菌株中特定有丝分裂周期损伤的方案，并描述了一个推定的有丝分裂周期突变体的特性。
