Sun B, Yuan Y, Zhang W, Che D
Department of Pathology, School of Basic Medical Sciences, Tongji Medical University, Wuhan.
Chin Med J (Engl). 1995 Nov;108(11):855-8.
The effects of hypoxic endothelial cell conditioned medium (HECCM) on proliferation and collagen synthesis of cultured porcine pulmonary arterial smooth muscle cells (PASMCs) were studied by 3H-thymidine (3H-TdR) and 3H-proline incorporations, image analysis for determination of DNA content and colorimetric assay using MTT, and the inhibitory effects of radix salviae miltiorrhizae (RSM) on them were also investigated. The results showed that HECCM could induce enhancement of the enzymatic activity of mitochondria, increase of the nucleic DNA content and increases of the 3H-TdR and 3H-proline incorporation in PASMCs. The 3H-proline incorporation in PASMCs cultured in HECCM was 1.83 times as much as that cultured in normoxic endothelial cell conditioned medium (NECCM). Compared with the control, Chinese herb medicine RSM could inhibit the proliferation of PASMCs cultured in HECCM and decrease the 3H-proline incorporation in PASMCs cultured in both HECCM and NECCM (P < 0.001). However, RSM had no effects on the nucleic DNA content and 3H-TdR incorporation into DNA of PASMCs cultured in NECCM. It suggests that hypoxia may stimulate the endothelia to synthesize and secrete some cytokines which can stimulate the proliferation and the synthesis of collagen of PASMCs and RSM can inhibit this process.
采用³H-胸腺嘧啶核苷（³H-TdR）掺入法、³H-脯氨酸掺入法、图像分析法测定DNA含量以及MTT比色法，研究了缺氧内皮细胞条件培养液（HECCM）对培养的猪肺动脉平滑肌细胞（PASMCs）增殖和胶原合成的影响，并探讨了丹参（RSM）对其的抑制作用。结果显示，HECCM可诱导PASMCs线粒体酶活性增强、核酸DNA含量增加以及³H-TdR和³H-脯氨酸掺入增加。在HECCM中培养的PASMCs的³H-脯氨酸掺入量是在常氧内皮细胞条件培养液（NECCM）中培养的1.83倍。与对照组相比，中药丹参可抑制在HECCM中培养的PASMCs的增殖，并降低在HECCM和NECCM中培养的PASMCs的³H-脯氨酸掺入量（P < 0.001）。然而，丹参对在NECCM中培养的PASMCs的核酸DNA含量和³H-TdR掺入DNA无影响。这表明缺氧可能刺激内皮细胞合成和分泌某些细胞因子，这些细胞因子可刺激PASMCs的增殖和胶原合成，而丹参可抑制这一过程。
