Huang B, Che D, Zhang W
Department of Pathology, Tongji Medical University, Wuhan.
Zhonghua Bing Li Xue Za Zhi. 1995 Oct;24(5):306-8.
The effects of hypoxia on phenotype modulation of pulmonary artery smooth muscle cells (PASMC) was observed using cell culture and morphometry analysis. The results showed that the number of diploid cells and alpha-sm-actin of the hypoxic endothelial cell condition medium group (HECCM) was lower and the myofibrils were even less than that of the normal endothelial cell condition medium group (NECCM). On the contrary, the volume density of rough endothelial reticulum (RER) and mitochondria of the HECCM group were higher than that of the NECCM group. However, under hypoxic condition for 24 hours, the phenotype of cultured PASMCs remained unchanged. It suggests that the HECCM can induce phenotype modulation of PASMCs, may be mediated by the pulmonary artery endothelial cells (PAECs) which can secrete some cytokines affecting the PASMCs.
采用细胞培养和形态计量学分析方法，观察缺氧对肺动脉平滑肌细胞（PASMC）表型调节的影响。结果显示，缺氧内皮细胞条件培养基组（HECCM）的二倍体细胞数量和α-平滑肌肌动蛋白含量低于正常内皮细胞条件培养基组（NECCM），且肌原纤维更少。相反，HECCM组粗面内质网（RER）和线粒体的体积密度高于NECCM组。然而，在缺氧24小时的条件下，培养的PASMCs表型未发生变化。这表明HECCM可诱导PASMCs的表型调节，可能是由可分泌某些影响PASMCs的细胞因子的肺动脉内皮细胞（PAECs）介导的。
