Guttman A, Pritchett T
Beckman Instruments, Inc., Fullerton, CA 92634, USA.
Electrophoresis. 1995 Oct;16(10):1906-11. doi: 10.1002/elps.11501601314.
In this paper we report the capillary gel electrophoresis separation of 1-aminopyrene-3,6,8-trisulfonic acid (APTS) labeled oligosaccharides, released enzymatically from bovine pancreatic ribonuclease B. The released and labeled high-mannose structures were identified by spiking the separated peaks with the appropriate commercially available individual oligosaccharides. Baseline separation of the three positional isomers of the mannose-7 and mannose-8 oligosaccharides was attained. Comparison of the electrophoretic mobilities of the high-mannose type branched carbohydrates to the linear molecules of maltooligosaccharides (glucose oligomers) have been shown using different gel concentrations in the running buffer system. We observed that increasing gel concentration in the running buffer causes an increase in the relative mobility values of the high-mannose type carbohydrate molecules compared to the linear glucose oligomers. Analysis of our data indicated that this increase in relative migration time was not due to sieving, but seemed to be related to the mannose content and hydrodynamic volume of the branched glycans as well as to the viscosity of the separation medium.
在本文中，我们报道了对从牛胰核糖核酸酶B酶解释放出的1-氨基芘-3,6,8-三磺酸（APTS）标记的寡糖进行毛细管凝胶电泳分离的结果。通过用适当的市售单寡糖对分离出的峰进行加样，鉴定出了释放并标记的高甘露糖结构。实现了甘露糖-7和甘露糖-8寡糖的三种位置异构体的基线分离。在运行缓冲液系统中使用不同的凝胶浓度，比较了高甘露糖型支链碳水化合物与麦芽寡糖（葡萄糖寡聚物）线性分子的电泳迁移率。我们观察到，与线性葡萄糖寡聚物相比，运行缓冲液中凝胶浓度的增加会导致高甘露糖型碳水化合物分子的相对迁移率值增加。对我们数据的分析表明，相对迁移时间的这种增加不是由于筛分，而是似乎与支链聚糖的甘露糖含量和流体力学体积以及分离介质的粘度有关。
