Bossi A, Righetti P G
Department of Cell Biology, University of Calabria, Arcavacata di Rende Cosenza.
Electrophoresis. 1995 Oct;16(10):1930-4. doi: 10.1002/elps.11501601319.
Multicompartment electrolyzers with isoelectric membranes can be successfully utilized for the preparation of carrier ampholytes of narrow pH ranges from commercially available wider pH intervals. An example is given on the preparation of a 0.6 pH unit (pH 6.7-7.3) range from a standard pH 6-8 interval. A 10% Ampholine solution is focused in an electrolyzer equipped with the following isoelectric membranes: pI 6.0, 6.7, 7.3 and 8.0. The narrow pH 6.7-7.3 cut can be efficiently utilized for base-line separation and quantitation of hemoglobin A from its glycated form, Hb A1c. This analysis is important for the screening and follow up of diabetic patients. The advantage of multicompartment electrolyzers are: (i) precision in the preparation of narrow pH cuts, due to the presence of membranes with defined pI values; (ii) ability to perform in both small- and large-scale operations and (iii) absence of contaminants leaching from granulated supports, as typical of some previous techniques.
带有等电膜的多隔室电解槽可成功用于从市售的较宽pH区间制备窄pH范围的载体两性电解质。给出了一个从标准pH 6 - 8区间制备0.6 pH单位（pH 6.7 - 7.3）范围的示例。将10%的两性电解质溶液在配备以下等电膜的电解槽中聚焦：pI 6.0、6.7、7.3和8.0。窄pH 6.7 - 7.3区间可有效地用于从糖化血红蛋白Hb A1c中基线分离和定量血红蛋白A。该分析对于糖尿病患者的筛查和随访很重要。多隔室电解槽的优点是：（i）由于存在具有确定pI值的膜，制备窄pH区间时精度高；（ii）能够进行小规模和大规模操作；（iii）不像一些先前技术那样存在从颗粒状载体中浸出污染物的情况。
