Hata H, Kuga T, Takeshita A
Research Institute of Angiocardiology and Cardiovascular Clinic, Faculty of Medicine, Kyushu University, Fukuoka.
Fukuoka Igaku Zasshi. 1995 Dec;86(12):437-42.
It is well known that leukocytes adherence to the endothelium of arterial wall occurs in diet-induced hypercholesterolemic animals. We examined the relationship between leukocytes adherence and atherosclerosis in the thoratic aorta of the Watanabe heritable hyperlipidemic rabbits (WHHL rabbits). Five of 2 or 3 months old WHHL rabbits were sacrificed and after perfusion fixation with 2.5% gulutaraldehyde, thoratic aorta was taken out carefully and divided into the 4 portions: Portion 1; cranial side of aortic arch, Portion 2; caudal side of aortic arch, Portion 3; upper side of thoratic aorta except aortic arch, Portion 4; lower side of thoratic aorta except aortic arch. Three to 6 samples from each portion except branching sites were examined using electron microscopy, and the counts of adherent leukocytes (LC) in each portion were calculated. Seven of 6 to 12 months old WHHL rabbits were sacrificed and the internal side of thoratic aorta was cut opened from the ventral side and the atherosclerotic lesions were copied. From these copies, the % area of atherosclerotic plaques (%AT) in each 4 portions as described was calculated using microcomputer. LC in Portion 1 to 4 was 265 +/- 62, 234 +/- 46, 53 +/- 8 and 41 +/- 13/mm2 respectively. LC in Portion 1 or 2 was significantly larger than that in Portion 3 or 4 (p < 0.05). The endothelium to which leukocytes adhered was intact. %AT in Portion 1 to 4 was 68 +/- 8, 63 +/- 8, 40 +/- 8 and 34 +/- 8% respectively. %AT in Portion 1 or 2 was significantly larger than that in Portion 3 or 4 (p < 0.05). It is concluded that leukocytes adherence to the intact endothelium of the arterial wall was one of the geneses and promoters of atherosclerosis in WHHL rabbits.
众所周知，在饮食诱导的高胆固醇血症动物中会发生白细胞黏附于动脉壁内皮的现象。我们研究了渡边遗传性高脂血症兔（WHHL兔）胸主动脉中白细胞黏附与动脉粥样硬化之间的关系。处死5只2至3月龄的WHHL兔，用2.5%戊二醛灌注固定后，小心取出胸主动脉并分为4部分：第1部分，主动脉弓头侧；第2部分，主动脉弓尾侧；第3部分，除主动脉弓外胸主动脉上侧；第4部分，除主动脉弓外胸主动脉下侧。除分支部位外，从每个部分取3至6个样本进行电子显微镜检查，并计算每个部分中黏附白细胞（LC）的数量。处死7只6至12月龄的WHHL兔，从腹侧切开胸主动脉内侧并复制动脉粥样硬化病变。利用微型计算机从这些复制品中计算上述4个部分中动脉粥样硬化斑块的面积百分比（%AT）。第1至4部分的LC分别为265±62、234±46、53±8和41±13/mm²。第1或2部分的LC显著大于第3或4部分（p<0.05）。白细胞黏附的内皮是完整的。第1至4部分的%AT分别为68±8、63±8、40±8和34±8%。第1或2部分的%AT显著大于第3或4部分（p<0.05）。得出的结论是，白细胞黏附于动脉壁完整内皮是WHHL兔动脉粥样硬化的发病机制和促进因素之一。
