重组牛生长激素（保饲灵）对两岁虹鳟（Oncorhynchus mykiss）生长性能和身体组成的剂量反应效应。
Dose-response effects of recombinant bovine somatotropin (Posilac) on growth performance and body composition of two-year-old rainbow trout (Oncorhynchus mykiss).
作者信息
Garber M J, DeYonge K G, Byatt J C, Lellis W A, Honeyfield D C, Bull R C, Schelling G T, Roeder R A
机构信息
Department of Animal and Veterinary Science, University of Idaho, Moscow 83844-2330, USA.
出版信息
J Anim Sci. 1995 Nov;73(11):3216-22. doi: 10.2527/1995.73113216x.
Two hundred rainbow trout (Oncorhynchus mykiss, mean weight 301.5 g) were allotted to four treatments with five replicates in a randomized block design to determine the dose-response effects of recombinant bovine somatotropin (rbST; Posilac) on growth performance and carcass composition. Treatments were sham-injected controls (S), 10 micrograms/g BW of rbST (L), 20 micrograms/g BW of rbST (M), and 30 micrograms/g BW of rbST (H). The tanks held 135 L; water flow = 15.1 L/min; temperature = 15 degrees C. The fish were maintained in a 12-h light:dark cycle and hand-fed twice daily. The fish received a single intraperitoneal (i.p.) injection on d 0 and were weighed on d 0, 14, 28, and 56. On d 56 the fish were killed. The whole body (WBW), eviscerated carcass (EC), viscera (VIS), and reproductive organ weights and the increase in average daily body length (ADL) were determined. Recombinant bST reduced (linear, P < .004) feed intake 17.6% from d 0 to 14 and improved ADG 44.8% from d 0 to 14 (linear, P < .001) and 8.1% from d 0 to 56 (linear, P < .022). Treated groups had improved (linear, P < .001) feed efficiencies for d 0 to 28. Treatment with rbST increased final weight (linear, P < .018) and length (linear, P < .001), decreased carcass dry matter (linear, P < .001) and fat (linear, P < .001), content, increased carcass ash (linear, P < .001) and tended to increase carcass protein (linear, P < .054) content. Recombinant bST increased WBW (linear, P < .018) and EC (linear, P < .003) but decreased (linear, P < .015) testes weight. Ovary weights, VIS and overall gonadosomatic index were unaffected (P > .05) by rbST. Recombinant bST was undetectable in serum samples taken on d 56 as determined by radioimmunoassay. Overall, the improved ADG, feed efficiency, body mass, and composition indicate that administration of rbST to rainbow trout may be an efficacious method of modulating growth in fish.
将200条虹鳟鱼（Oncorhynchus mykiss，平均体重301.5克）按照随机区组设计分配到四种处理组，每组五个重复，以确定重组牛生长激素（rbST；Posilac）对生长性能和胴体组成的剂量反应效应。处理组分别为假注射对照组（S）、10微克/克体重的rbST组（L）、20微克/克体重的rbST组（M）和30微克/克体重的rbST组（H）。养殖水箱容量为135升；水流速度 = 15.1升/分钟；水温 = 15摄氏度。鱼饲养在12小时光照：12小时黑暗的循环环境中，每天人工投喂两次。鱼在第0天接受一次腹腔注射，在第0天、14天、28天和56天称重。在第56天处死鱼。测定全鱼体重（WBW）、去内脏胴体（EC）、内脏（VIS）、生殖器官重量以及平均日体长增加量（ADL）。重组bST使第0天至14天的采食量降低了17.6%（呈线性关系，P < 0.004），使第0天至14天的平均日增重提高了44.8%（呈线性关系，P < 0.001），第0天至56天提高了8.1%（呈线性关系，P < 0.022）。处理组第0天至28天的饲料效率有所提高（呈线性关系，P < 0.001）。用rbST处理可增加最终体重（呈线性关系，P < 0.018）和体长（呈线性关系，P < 0.001），降低胴体干物质（呈线性关系，P < 0.001）和脂肪（呈线性关系，P < 0.001）含量，增加胴体灰分（呈线性关系，P < 0.001），并倾向于增加胴体蛋白质含量（呈线性关系，P < 0.054）。重组bST增加了全鱼体重（呈线性关系，P < 0.018）和去内脏胴体重量（呈线性关系，P < 0.003），但降低了（呈线性关系，P < 0.015）睾丸重量。卵巢重量、内脏重量和总体性腺指数不受rbST影响（P > 0.05）。通过放射免疫测定法测定，在第56天采集的血清样本中未检测到重组bST。总体而言，虹鳟鱼平均日增重、饲料效率、体重和组成的改善表明，对虹鳟鱼施用rbST可能是调节鱼类生长的一种有效方法。
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