美国一株产β-D-葡萄糖醛酸酶的O157:H7血清型大肠杆菌的分离与鉴定
Isolation and characterization of a beta-D-glucuronidase-producing strain of Escherichia coli serotype O157:H7 in the United States.
作者信息
Hayes P S, Blom K, Feng P, Lewis J, Strockbine N A, Swaminathan B
机构信息
Foodborne and Diarrheal Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 1995 Dec;33(12):3347-8. doi: 10.1128/jcm.33.12.3347-3348.1995.
Abstract
A phenotypic variant of Escherichia coli serotype O157:H7 (G5101) was isolated from a patient with bloody diarrhea. Strain G5101 does not ferment sorbitol but is beta-D-glucuronidase and urease positive. Serotyping and colony hybridization using a serotype-specific DNA probe confirmed that the isolate was O157:H7. G5101 produces Shiga-like toxins I and II and contains an eae gene that is highly conserved in the O157:H7 serotype. This strain would have been missed by laboratories that screen for the sorbitol-negative, beta-D-glucuronidase-negative phenotype in isolating E. coli O157:H7 from clinical and food specimens.
摘要
从一名血性腹泻患者中分离出大肠杆菌O157:H7血清型（G5101）的一个表型变异株。G55菌株G5101不发酵山梨醇，但β-D-葡萄糖醛酸酶和脲酶呈阳性。使用血清型特异性DNA探针进行血清分型和菌落杂交证实该分离株为O157:H7。G5101产生志贺样毒素I和II，并含有一个在O157:H7血清型中高度保守的eae基因。在从临床和食品标本中分离大肠杆菌O157:H7时，那些筛查山梨醇阴性、β-D-葡萄糖醛酸酶阴性表型的实验室可能会遗漏该菌株。
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