Gunzer F, Böhm H, Rüssmann H, Bitzan M, Aleksic S, Karch H
Institut für Hygiene und Mikrobiologie, Universität Würzburg, Germany.
J Clin Microbiol. 1992 Jul;30(7):1807-10. doi: 10.1128/jcm.30.7.1807-1810.1992.
Shiga-like toxin-producing Escherichia coli strains of serogroup O157 were identified in 26 of 104 patients with hemolytic-uremic syndrome and in 18 of 668 patients with diarrhea. All strains were identified by colony hybridization with DNA probes complementary to Shiga-like toxin I and Shiga-like toxin II gene sequences and characterized by biochemical tests and serotyping. Seventeen of these 44 patients had E. coli O157 strains which were unusual because they fermented sorbitol within 24 h of incubation and were positive for beta-glucuronidase activity. Culture filtrates of these sorbitol-fermenting strains were highly toxic to Vero cells in culture. Serological tests and DNA analysis performed by restriction endonuclease digestion of B-subunit toxin genes revealed that all 17 isolates produced Shiga-like toxin II. Although by using molecular probes we established a high frequency of sorbitol-fermenting E. coli O157 strains in the patients we examined, further studies on the prevalence of such isolates in other areas of endemic disease are clearly warranted.
在104例溶血尿毒综合征患者中，有26例以及668例腹泻患者中有18例被鉴定出感染了产志贺样毒素的O157血清群大肠杆菌菌株。所有菌株均通过与志贺样毒素I和志贺样毒素II基因序列互补的DNA探针进行菌落杂交鉴定，并通过生化试验和血清分型进行表征。这44例患者中有17例的大肠杆菌O157菌株较为特殊，因为它们在培养24小时内就能发酵山梨醇，且β-葡萄糖醛酸酶活性呈阳性。这些发酵山梨醇的菌株的培养滤液对培养中的Vero细胞具有高毒性。通过对B亚单位毒素基因进行限制性内切酶消化进行的血清学检测和DNA分析表明，所有17株分离株均产生志贺样毒素II。尽管通过使用分子探针，我们在所检测的患者中发现了较高频率的发酵山梨醇的大肠杆菌O157菌株，但显然有必要对其他地方病流行地区此类分离株的流行情况进行进一步研究。
