Jeffery N D, Blakemore W F
J Neurocytol. 1995 Oct;24(10):775-81. doi: 10.1007/BF01191213.
Local injections of lysolecithin are commonly used to produce areas of demyelination in the CNS. For the mouse, the demyelinating initial phase of lesion development has been described previously but the spontaneous repair which follows has not. In this study we describe the morphological sequelae which result from the injection of 2 microliters of 1% lysolecithin into the spinal cord of adult mice. Lesion length was variable, extending 8 mm or more in 33% of lesioned animals. By 7 days after injection very little extracellular myelin debris was detected and remyelination had commenced. Remyelination progressed rapidly so that almost all axons were invested by myelin sheaths by 23 days. Remyelination was accompanied by a prominent astrocytosis. The long lesion length and the rapidity of repair has important implications for studies designed to assess the ability of transplanted myelinogenic cells to migrate towards demyelinating lesions.
局部注射溶血卵磷脂常用于在中枢神经系统中产生脱髓鞘区域。对于小鼠，此前已描述过损伤发展的脱髓鞘初始阶段，但后续的自发修复过程尚未有相关描述。在本研究中，我们描述了向成年小鼠脊髓注射2微升1%溶血卵磷脂所导致的形态学后遗症。损伤长度各不相同，33%的损伤动物中损伤长度延伸达8毫米或更长。注射后7天，几乎检测不到细胞外髓鞘碎片，并且再髓鞘化已经开始。再髓鞘化进展迅速，以至于到23天时几乎所有轴突都被髓鞘包裹。再髓鞘化伴随着显著的星形细胞增生。损伤长度较长以及修复速度较快对于旨在评估移植的髓鞘形成细胞向脱髓鞘损伤部位迁移能力的研究具有重要意义。
