纤连蛋白可诱导培养的类风湿性滑膜细胞中蛋白酶依赖性的局灶性基质消耗和细胞重叠。
Fibronectin induces protease dependent focal matrix depletion and cell overlap in cultured rheumatoid synoviocytes.
作者信息
Wang A Z, Zhang X R, Wang J C, Fisher G W, Diamond H S
机构信息
Department of Medicine, Western Pennsylvania Hospital, Pittsburgh 15224, USA.
出版信息
J Rheumatol. 1995 May;22(5):817-28.
OBJECTIVE
To characterize the effect of added fibronectin (Fn) on human rheumatoid synoviocytes (RAS).
METHODS
Early passage cultured RAS were studied by fluorescent imaging microscopy with multiple parameter overlaying and confocal laser scanning microscopy.
RESULTS
Added Fn induced a localized decrease in matrix Fn at sites of cell overlap. Similar matrix depletion could be induced by collagen VI, cell binding (120 k) and heparin binding (60 k) fragments of Fn, but not by gelatin binding fragment (45 k).
CONCLUSION
The decrease in matrix Fn was associated with the induction of a transformation-like phenotype of overlapping cell growth. Both phenomena were inhibited by serine protease inhibitors.
目的
表征添加纤连蛋白（Fn）对人类风湿性滑膜细胞（RAS）的影响。
方法
通过多参数叠加荧光成像显微镜和共聚焦激光扫描显微镜对早期传代培养的RAS进行研究。
结果
添加Fn导致细胞重叠部位的基质Fn局部减少。胶原蛋白VI、Fn的细胞结合（120k）和肝素结合（60k）片段可诱导类似的基质消耗，但明胶结合片段（45k）则不能。
结论
基质Fn的减少与重叠细胞生长的转化样表型的诱导有关。这两种现象均被丝氨酸蛋白酶抑制剂抑制。
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