Erlacher L, Maier R, Ullrich R, Kiener H, Aringer M, Menschik M, Graninger W
Department of Internal Medicine III, University of Vienna, Austria.
J Rheumatol. 1995 May;22(5):926-31.
To investigate the expression of bcl-2 in healthy and osteoarthritic articular cartilage at the transcriptional level and at the protein level.
bcl-2 mRNA was detected by reverse transcriptase/polymerase chain reaction. The expression of bcl-2 protein was studied using flow cytometry analysis with a monoclonal antibody against intracellular bcl-2 and by immunohistochemistry.
mRNA for bcl-2 was detectable in chondrocytes of both healthy and osteoarthritic cartilage. bcl-2 protein was present in the chondrocytes of the middle layer of cartilage by immunohistochemical staining. In osteoarthritis (OA), chondrocytes adjacent to cartilage defects expressed a particularly high signal intensity. Flow cytometry analysis demonstrated the presence of bcl-2 protein in virtually all chondrocytes.
Our results show that bcl-2 is expressed in healthy adult and osteoarthritic human cartilage. The increased staining of chondrocytes adjacent to osteoarthritic defects indicates a differential regulation of programmed cell death during the degenerative process in OA.
在转录水平和蛋白质水平研究bcl-2在健康及骨关节炎关节软骨中的表达。
采用逆转录/聚合酶链反应检测bcl-2 mRNA。使用抗细胞内bcl-2的单克隆抗体通过流式细胞术分析和免疫组织化学研究bcl-2蛋白的表达。
在健康和骨关节炎软骨的软骨细胞中均可检测到bcl-2的mRNA。通过免疫组织化学染色，bcl-2蛋白存在于软骨中层的软骨细胞中。在骨关节炎（OA）中，与软骨缺损相邻的软骨细胞表达出特别高的信号强度。流式细胞术分析表明几乎所有软骨细胞中都存在bcl-2蛋白。
我们的结果表明bcl-2在健康成人及骨关节炎人类软骨中表达。骨关节炎缺损相邻软骨细胞染色增加表明在OA退变过程中程序性细胞死亡存在差异调节。
