Schulze E, Schulze B
Institute of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH 44106-4943, USA.
J Mol Evol. 1995 Dec;41(6):833-40. doi: 10.1007/BF00173162.
We investigated the evolutionary history of the divergent vertebrate linker histones H1 zero, H5, and H1M. We observed that the sequence of the central conserved domain of these vertebrate proteins shares characteristic features with histone H1 proteins of plants and invertebrate animals which otherwise never appear in any vertebrate histone H1 protein. A quantitative analysis of 58 linker histone sequences also reveals that these proteins are more similar to invertebrate and plant histone H1 than to histone H1 of vertebrates. A phylogenetic tree deduced from an alignment of the central domain of all known linker histones places H1 zero, H5, and H1M in close vicinity to invertebrate sperm histone H1 proteins and to invertebrate histone H1 proteins encoded by polyadenylated mRNAs. We therefore conclude that the ancestors of the vertebrate linker histones H1 zero, H5, and H1M diverged from the main group of histone H1 proteins before the vertebrate type of histone H1 was established in evolution. We discuss this observation in the general context of linker histone evolution.
我们研究了脊椎动物中分化的连接组蛋白H1零、H5和H1M的进化历史。我们观察到，这些脊椎动物蛋白的中央保守结构域序列与植物和无脊椎动物的组蛋白H1蛋白具有共同特征，而这些特征在任何脊椎动物组蛋白H1蛋白中都从未出现过。对58个连接组蛋白序列的定量分析还表明，这些蛋白与无脊椎动物和植物组蛋白H1的相似性高于与脊椎动物组蛋白H1的相似性。从所有已知连接组蛋白中央结构域的比对推导的系统发育树将H1零、H5和H1M置于与无脊椎动物精子组蛋白H1蛋白以及由多聚腺苷酸化mRNA编码的无脊椎动物组蛋白H1蛋白的附近。因此，我们得出结论，脊椎动物连接组蛋白H1零、H5和H1M的祖先在进化中脊椎动物型组蛋白H1建立之前就已从组蛋白H1蛋白的主要群体中分化出来。我们在连接组蛋白进化的一般背景下讨论这一观察结果。
