除H1(0)基因外，所有已知的人类H1组蛋白基因都聚集在6号染色体上。

All known human H1 histone genes except the H1(0) gene are clustered on chromosome 6.

作者信息

Albig W, Drabent B, Kunz J, Kalff-Suske M, Grzeschik K H, Doenecke D

机构信息

Institut für Biochemie und Molekulare Zellbiologie, Universität Göttingen, Germany.

出版信息

Genomics. 1993 Jun;16(3):649-54. doi: 10.1006/geno.1993.1243.

DOI:10.1006/geno.1993.1243

PMID:8325638

Abstract

PCR analysis of chromosomal DNA from a panel of human-rodent somatic cell hybrids revealed that the five human H1 histone genes H1.1 to H1.5 and the gene encoding the testis-specific H1t subtype, all of which form clusters with core histone genes, are located on chromosome 6. The H1(0) subtype, which is not neighbored by core histone genes, maps to chromosome 22. Fluorescence in situ hybridization with human metaphase chromosomes and PCR analysis of somatic cell hybrid DNA carrying only fragments of chromosome 6 revealed the region 6p21.1 to 6p22.2 as the histone gene cluster region.

摘要

对一组人-啮齿动物体细胞杂种的染色体DNA进行PCR分析发现，5个人类H1组蛋白基因H1.1至H1.5以及编码睾丸特异性H1t亚型的基因，所有这些基因都与核心组蛋白基因形成簇，位于6号染色体上。不与核心组蛋白基因相邻的H1(0)亚型定位于22号染色体。用人中期染色体进行荧光原位杂交以及对仅携带6号染色体片段的体细胞杂种DNA进行PCR分析，揭示了6p21.1至6p22.2区域为组蛋白基因簇区域。