Wang H, Nuttall P A
NERC Institute of Virology and Environmental Microbiology, Oxford, UK.
Parasite Immunol. 1995 Oct;17(10):517-24. doi: 10.1111/j.1365-3024.1995.tb00882.x.
Three abundant immunoglobulin G binding proteins (IGBPs) of 29 kDa (IGBP-MA), 25 kDa (IGBP-MB), and 21 kDa (IGBP-MC) were isolated from partly fed male Rhipicephalus appendiculatus ticks using an agarose-IgG column. After separating the proteins by SDS-PAGE, each individual IGBP band was cut out from the gel and used to raise antiserum in guinea pigs. Using immunoblotting, each of the three IGBPs was shown to be antigenically distinct, and specific for feeding male R. appendiculatus ticks. They were detected in the salivary glands of male ticks that had been feeding in either the presence of absence of female ticks, and appeared to be glycoproteins. The smallest protein, IGBP-MC, bound to guinea pig IgG (on which species the ticks were fed) and also bound to human and bovine IgG, whereas IGBP-MA and possibly IGBP-MB only bound to guinea pig IgG. The function of IGBPs in tick salivary glands and their significance in male ticks are discussed.
利用琼脂糖 - IgG柱从部分进食的雄性微小牛蜱中分离出三种丰富的免疫球蛋白G结合蛋白（IGBPs），分别为29 kDa（IGBP - MA）、25 kDa（IGBP - MB）和21 kDa（IGBP - MC）。通过SDS - PAGE分离蛋白质后，从凝胶中切下每条单独的IGBP条带，并用于在豚鼠中制备抗血清。通过免疫印迹法表明，这三种IGBPs中的每一种在抗原性上都是不同的，并且对进食的雄性微小牛蜱具有特异性。在有或没有雌性蜱存在的情况下进食的雄性蜱的唾液腺中都检测到了它们，并且它们似乎是糖蛋白。最小的蛋白质IGBP - MC与豚鼠IgG（蜱以此物种为食）结合，也与人及牛的IgG结合，而IGBP - MA以及可能的IGBP - MB仅与豚鼠IgG结合。文中讨论了IGBPs在蜱唾液腺中的功能及其在雄性蜱中的意义。
